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PMID: 6283523 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 8 ·1982-04-00 ·Pages 2437-41

Robert B, Weydert A, Caravatti M, Minty A, Cohen A, Daubas P, Gros F, Buckingham M

Abstract

A recombinant plasmid with a cDNA sequence transcribed from mouse skeletal muscle RNA is shown to hybridize with mRNAs for myosin light chains LC1F and LC3F. The inserted fragment corresponds exclusively to the 3'-noncoding region of the mRNA. It hybridizes almost exclusively with the two light chain messengers from fast skeletal muscle RNA of adult mouse. Slight hybridization is seen with RNA from heart muscle and embryonic skeletal muscle. The implications of the conservation of the 3'-noncoding regions between the two mRNAs are discussed.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Mice Muscles/metabolism Myosin Subfragments Myosins/genetics Nucleic Acid Hybridization Peptide Fragments/genetics Plasmids RNA, Messenger/genetics
Chemicals
Myosin Subfragments Peptide Fragments RNA, Messenger DNA DNA Restriction Enzymes Myosins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Robert B
Weydert A
Caravatti M
Minty A
Cohen A
Daubas P
Gros F
Buckingham M
References (35)
35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.8.2437
Published
1982-04-00
Pages
2437-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346213
Subset
IM
Databases
GENBANK
K02242
External Links
PubMed source DOI: 10.1073/pnas.79.8.2437 PMC Full Text View journal details
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