Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 487-506

Smith DF, Searle PF, Williams JG

Abstract

A 1700 nucleotide DNA sequence derived from Xenopus vitellogenin mRNA has been cloned in the bacterial plasmid pBR322. The identity of the cloned sequence was verified in two ways. Firstly, the plasmid DNA was shown to hybridise to an RNA of the correct size (6,700 nucleotides). This was shown by in situ hybridisation to electrophoretically separated RNA and also by the formation of "R-loops" with purified vitellogenin mRNA. Then, using a novel procedure in which plasmid DNA covalently bound to diazotised paper is used to select complementary mRNA sequences, the cloned sequence was shown to hybridise to an mRNA which directed the synthesis of vitellogenin when translated in a reticulocyte lysate cell-free system.

MeSH Terms

Animals Base Sequence DNA, Recombinant Kinetics Male Molecular Weight Nucleic Acid Hybridization Plasmids Protein Biosynthesis RNA, Messenger/metabolism Vitellogenins/biosynthesis Xenopus

Chemicals

DNA, Recombinant RNA, Messenger Vitellogenins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Smith D F

Searle P F

Williams J G

References (25)

25 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.2.487

Published

1979-02-00

Pages

487-506

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327709

Subset

External Links

PubMed source DOI: 10.1093/nar/6.2.487 PMC Full Text View journal details

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Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA.

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