Home LiteratureArticle Details
PMID: 6278417 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 3' co-terminus of two early herpes simplex virus type 1 mRNAs.

Nucleic acids research ·Vol. 10 ·No. 2 ·1982-01-22 ·Pages 501-12

McLauchlan J, Clements JB

Abstract

A 3' co-terminus of two early herpes simplex virus type 1 mRNAs has been identified using the nuclease -S1 mapping procedure with cloned virus DNA probes. These mRNAs (5.0 kb and 1.2 kb), located within the genome region 0.56-0.60, are unspliced and are transcribed rightwards on the prototype genome orientation. The position of their 3' ends has been located on the virus DNA sequence and lies downstream from the polyadenylation signal 5'-AATAAA-3'. This hexanucleotide sequence also was present in the complementary DNA strand and was shown to be the polyadenylation signal for a leftwards-transcribed late mRNA. The abundance within the cytoplasm of the 5.0 kb and 1.2 kb mRNAs was investigated. Results indicated that these mRNAs were regulated in concert. It is suggested that sequences at the 3' co-terminus may be involved in their regulation.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism DNA, Viral/genetics RNA Splicing RNA, Messenger/genetics Simplexvirus/genetics Transcription, Genetic
Chemicals
DNA, Recombinant DNA, Viral RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McLauchlan J
Clements J B
References (23)
23 references, click to expand
  1. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  2. Sequence arrangement in herpes simplex virus type 1 DNA: identification of terminal fragments in restriction endonuclease digests and evidence for inversions in redundant and unique sequences.
    J Virol. 1976 Oct;20(1):211-21 PMID: 185412
  3. Temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genome.
    Cell. 1977 Sep;12(1):275-85 PMID: 198141
  4. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  5. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  6. Sequence of chicken ovalbumin mRNA.
    Nature. 1978 Jun 29;273(5665):723-8 PMID: 661981
  7. Orientation of herpes simplex virus type 1 immediate early mRNA's.
    Nucleic Acids Res. 1979 Sep 11;7(1):77-91 PMID: 226944
  8. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  9. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  10. Structure and genomic organization of the mouse dihydrofolate reductase gene.
    Cell. 1980 Feb;19(2):355-64 PMID: 6244105
  11. The gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA.
    Cell. 1980 Mar;19(3):671-81 PMID: 6985473
  12. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  13. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.
    Cell. 1980 Aug;21(1):179-87 PMID: 6157477
  14. Transcription and RNA processing by the DNA tumour viruses.
    Nature. 1980 Oct 9;287(5782):491-9 PMID: 6252477
  15. Temporal regulation of herpes simplex virus type 2 transcription and characterization of virus immediate early mRNA's.
    Nucleic Acids Res. 1980 Jun 25;8(12):2627-45 PMID: 6253886
  16. Regulation of adenovirus-2 gene expression at the level of transcriptional termination and RNA processing.
    Nature. 1981 Mar 12;290(5802):113-8 PMID: 7207591
  17. The sequence of the 3' non-coding region of the hexon mRNA discloses a novel adenovirus gene.
    Nucleic Acids Res. 1981 Jan 10;9(1):1-17 PMID: 6259616
  18. Structures of two spliced herpes simplex virus type 1 immediate-early mRNA's which map at the junctions of the unique and reiterated regions of the virus DNA S component.
    J Virol. 1981 Jan;37(1):431-44 PMID: 6260993
  19. Detailed characterization of the mRNA mapping in the HindIII fragment K region of the herpes simplex virus type 1 genome.
    J Virol. 1981 Mar;37(3):1011-27 PMID: 6262521
  20. Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1441-5 PMID: 6262799
  21. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  22. Multiple polyadenylation sites in a mouse alpha-amylase gene.
    Nucleic Acids Res. 1981 May 25;9(10):2313-23 PMID: 6166922
  23. Nucleotide sequences of the joint between the L and S segments of herpes simplex virus types 1 and 2.
    J Gen Virol. 1981 Aug;55(Pt 2):315-31 PMID: 6270266
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.2.501
Published
1982-01-22
Pages
501-12
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326153
Subset
IM
Databases
GENBANK
J02214
External Links
PubMed source DOI: 10.1093/nar/10.2.501 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2