Home LiteratureArticle Details
PMID: 6269088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and mapping of the chloroplast DNA sequences for two messenger RNAs from mustard (Sinapis alba L.).

Nucleic acids research ·Vol. 9 ·No. 15 ·1981-08-11 ·Pages 3681-94

Link G

Abstract

Restriction fragments of chloroplast (cp)DNA from mustard leaves were cloned in E. coli using pBR 322 as the vehicle. Cloned fragments containing the structural sequences for two polypeptides of 56,000 and 35,000 daltons were selected by a mRNA hybridization-translation procedure. The cloned mustard genes are structurally related to chloroplast genes from maize. They hybridize to the maize mRNAs for the large subunit of ribulosebisphosphate carboxylase and for the 34,500 dalton precursor to a membrane protein. The coding sequence on mustard cpDNA for the 56,000 dalton polypeptide is colinear with a 1,500 base pair transcript, and the sequence for the 35,000 dalton polypeptide is colinear with a 1,220 base pair transcript.

MeSH Terms
Base Sequence Carboxy-Lyases/genetics Chloroplasts/metabolism Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/genetics Molecular Weight Nucleic Acid Hybridization Plants/genetics Plasmids Protein Biosynthesis RNA, Messenger/genetics Ribulose-Bisphosphate Carboxylase/genetics Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Messenger DNA Restriction Enzymes Carboxy-Lyases Ribulose-Bisphosphate Carboxylase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Link G
References (32)
32 references, click to expand
  1. Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1922-6 PMID: 168582
  2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  3. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  4. Location of the single gene for the large subunit of ribulosebisphosphate carboxylase on the maize chloroplast chromosome.
    J Biol Chem. 1979 Feb 10;254(3):905-10 PMID: 762099
  5. Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5487-91 PMID: 16592473
  6. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  7. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  8. Isolation, characterization and restriction endonuclease mapping of the Petunia hybrida chloroplast DNA.
    Nucleic Acids Res. 1981 Feb 11;9(3):503-17 PMID: 6261223
  9. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.
    Nucleic Acids Res. 1977;4(5):1539-52 PMID: 19730
  10. Characterization and transcription analysis of a cloned sequence derived from a major developmentally regulated mRNA of D. discoideum.
    Cell. 1979 Aug;17(4):903-13 PMID: 487435
  11. Proteins of cytoplasmic, chloroplast, and mitochondrial ribosomes of some plants.
    Biochim Biophys Acta. 1971 Jan 28;228(2):492-502 PMID: 4993620
  12. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  13. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  14. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  15. Plastid DNA-the plastome.
    Results Probl Cell Differ. 1980;10:45-96 PMID: 6999574
  16. Differential expression of the gene for the large subunit of ribulose bisphosphate carboxylase in maize leaf cell types.
    Cell. 1978 Nov;15(3):725-31 PMID: 728987
  17. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  18. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  19. Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA.
    Gene. 1979 Aug;6(4):285-306 PMID: 499808
  20. Rat insulin genes: construction of plasmids containing the coding sequences.
    Science. 1977 Jun 17;196(4296):1313-9 PMID: 325648
  21. Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardii.
    Cell. 1978 Oct;15(2):661-70 PMID: 719757
  22. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  23. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  24. Physical mapping of the ribosomal DNA region of Euglena gracilis chloroplast DNA.
    Eur J Biochem. 1978 Jul 17;88(1):127-34 PMID: 97079
  25. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  26. Construction of a SalI/PstI restriction map of spinach chloroplast DNA using low-gelling-temperature-agarose electrophoresis.
    Gene. 1980 Jan;8(2):179-91 PMID: 6244211
  27. Characterization of the gene and messenger RNA of the large subunit of ribulose 1,5-diphosphate carboxylase in Chlamydomonas reinhardii.
    J Mol Biol. 1979 Sep 25;133(3):417-34 PMID: 537056
  28. Mapping of rRNA genes in an inverted repeat in Nicotiana tabacum chloroplast DNA.
    Nucleic Acids Res. 1980 Aug 25;8(16):3505-16 PMID: 6253903
  29. Sizes, locations, and directions of transcription of two genes on a cloned maize chloroplast DNA sequence.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1832-6 PMID: 16592800
  30. Zea mays chloroplast ribosomal RNA genes are part of a 22,000 base pair inverted repeat.
    Cell. 1977 Aug;11(4):739-49 PMID: 890739
  31. Euglena gracilis chloroplast ribosomal RNA transcription units. I. The location of transfer RNA, 5 S, 16 S, and 23 S ribosomal RNA genes.
    J Biol Chem. 1980 Nov 25;255(22):10991-6 PMID: 6253496
  32. Maize plastid gene expressed during photoregulated development.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3060-4 PMID: 16592541
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.15.3681
Published
1981-08-11
Pages
3681-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327384
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.15.3681 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2