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PMID: 6268936 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cosmid cloning and transposon mutagenesis in Salmonella typhimurium using phage lambda vehicles.

Molecular & general genetics : MGG ·Vol. 181 ·No. 2 ·1981-00-00 ·Pages 153-7

Palva ET, Liljeström P, Harayama S

Abstract

We have constructed a strain of Salmonella typhimurium which contains the malB region from Escherichia coli and carries the bacteriophage lambda receptor protein in its outer membrane. Phage lambda adsorbs to this strain but cannot grow, thus providing a very useful system for transposon mutagenesis of S. typhimurium using lambda vehicles carrying transposons. This system can also be used for cosmid cloning.

MeSH Terms
Bacterial Outer Membrane Proteins Bacteriophage lambda/genetics Cloning, Molecular DNA Transposable Elements Escherichia coli/genetics F Factor Genetic Vectors Maltose/metabolism Mutation Plasmids Porins Receptors, Virus/genetics Salmonella typhimurium/genetics Transduction, Genetic
Chemicals
Bacterial Outer Membrane Proteins DNA Transposable Elements Porins Receptors, Virus maltoporins Maltose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Palva E T
Liljeström P
Harayama S
References (26)
26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268420
Published
1981-00-00
Pages
153-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268420
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