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PMID: 6268611 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tetracycline resistance transposon Tn1721: recA-dependent gene amplification and expression of tetracycline resistance.

Journal of bacteriology ·Vol. 147 ·No. 3 ·1981-09-00 ·Pages 851-9

Wiebauer K, Schraml S, Shales SW, Schmitt R

Abstract

The 7.1-megadalton transposon Tn1721 codes for inducible tetracycline resistance (Tcr). The transposable element consists of a "minor transposon" (3.6 megadaltons) encoding functions required for transposition and a "tet region" (3.5 megadaltons) encoding resistance. Multiple tandem repeats of the tet region can be generated by recA-dependent gene amplification. This feature of Tn1721 has been used to analyze the relationship between gene dosage and Tcr. Derivatives of plasmid R388:Tn1721 containing from one to nine copies of the tet region were isolated and separately transformed into recA host cells, where they are stably maintained. The results of the study of Tcr in these strains were as follows: (i) the uninduced, "basal" level of Tcr was linearly related to gene dosage between 4 and 36 copies of tet per chromosome equivalent; (ii) the underlying mechanism could not be attributed to reduced accumulation of the drug; and (iii) induction with tetracycline elicited a four- to fivefold reduction in drug accumulation, independent of the gene dosage.

MeSH Terms
DNA Transposable Elements Escherichia coli/drug effects,genetics Gene Amplification Genes, Bacterial Recombination, Genetic Tetracycline/metabolism,pharmacology
Chemicals
DNA Transposable Elements Tetracycline
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wiebauer K
Schraml S
Shales S W
Schmitt R
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.3.851-859.1981
Published
1981-09-00
Pages
851-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216121
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.147.3.851-859.1981 PMC Full Text View journal details
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