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PMID: 109839 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A genetic approach to analysis of transposons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 5 ·1979-05-00 ·Pages 2376-80

Beck CF

Abstract

Integration of the tetracycline resistance transposon Tn10 into lacI of a lacI-lacZ gene fusion permits the isolation of deletions that excise DNA from one end of Tn10 and fuse Tn10 genes with lacZ in such a manner that chimeric proteins with beta-galactosidase activity are produced. The synthesis of the chimeric proteins is under the same control as the transposon genes. Thus, regulation of expression of Tn10 genes can be investigated by measuring beta-galactosidase activity. Analysis of Tn10-lacZ fusions revealed different deletion endpoints within Tn10; lacZ has been fused to at least three different Tn10 genes or operons. Two of these genes are under the control of a tetracycline repressor.

MeSH Terms
Chromosome Deletion Drug Resistance, Microbial Escherichia coli/genetics Genes Genes, Regulator Hot Temperature Lac Operon Mutation Salmonella typhimurium/genetics Tetracycline/pharmacology Translocation, Genetic beta-Galactosidase/genetics
Chemicals
beta-Galactosidase Tetracycline
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Beck C F
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.5.2376
Published
1979-05-00
Pages
2376-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383604
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.5.2376 PMC Full Text View journal details
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