Home LiteratureArticle Details
PMID: 6262762 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Movement and site selection for priming by the primosome in phage phi X174 DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 2 ·1981-02-00 ·Pages 707-11

Arai K, Low RL, Kornberg A

Abstract

The Escherichia coli priming system used for initiation of DNA chains on phage phi X174 single-stranded DNA is a multiprotein unit called the primosome [Arai, K. & Kornberg. A. (1981) Proc. Natl. Acad. Sci. USA 78, 69-73]. Assembled with participation of seven prepriming proteins and primase at a unique place on the phi X174 DNA template, the primosome is bound tightly to the DNA, yet moves rapidly and unidirectionally opposite to primer and DNA chain synthesis. Contributions of protein n' and dnaB protein, two components of the primosome, to movement and site selection for priming are considered in this report. Figuratively, the primosome can be likened to a locomotive that depends on protein n' as its engine and dnaB protein as the engineer. Protein n', a DNA-dependent ATPase (dATPase) appears to use the energy of hydrolysis of the nucleoside triphosphate for processive translocation of the primosome. dnaB protein, A DNA-dependent ribonucleosidetriphosphatase, depends on allosteric effects of a nucleoside triphosphate to induce changes in the structure of the single-stranded DNA at preferred sequences that enable primase to synthesize a short primer for initiation of DNA synthesis (unpublished data). These primosome properties have important implications for the progress of the replication fork of the E. coli chromosome.

MeSH Terms
Adenosine Triphosphatases/metabolism Bacterial Proteins/metabolism Bacteriophage phi X 174/metabolism DNA/metabolism DNA Primase DNA Replication DNA, Viral/biosynthesis Escherichia coli/metabolism Genes, Viral Phosphoric Monoester Hydrolases/metabolism RNA Nucleotidyltransferases/metabolism Templates, Genetic Virus Replication
Chemicals
Bacterial Proteins DNA, Viral DNA DNA Primase RNA Nucleotidyltransferases Phosphoric Monoester Hydrolases Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Arai K
Low R L
Kornberg A
References (34)
34 references, click to expand
  1. Replication of duplex DNA by bacteriophage T7 DNA polymerase and gene 4 protein is accompanied by hydrolysis of nucleoside 5'-triphosphates.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1525-9 PMID: 323856
  2. Unique primed start of phage phi X174 DNA replication and mobility of the primosome in a direction opposite chain synthesis.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):69-73 PMID: 6454139
  3. Polyuridylic acid binding and translating by Escherichia coli ribosomes: stimulation by protein I, inhibition by aurintricarboxylic acid.
    Biochim Biophys Acta. 1972 Oct 27;281(3):381-92 PMID: 4565240
  4. Initiation of deoxyribonucleic acid synthesis. VII. A unique location of the gap in the M13 replicative duplex synthesized in vitro.
    J Biol Chem. 1974 May 25;249(10):3049-54 PMID: 4598119
  5. The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.
    J Biol Chem. 1975 Mar 25;250(6):1972-80 PMID: 1090613
  6. Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):921-5 PMID: 1093174
  7. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  8. Isolation of an intermediate which precedes dnaG RNA polymerase participation in enzymatic replication of bacteriophage phi X174 DNA.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):752-6 PMID: 768984
  9. Bacteriophage-T7-induced DNA-priming protein. A novel enzyme involved in DNA replication.
    Eur J Biochem. 1977 Feb;72(3):543-58 PMID: 320003
  10. dnaB protein of Escherichia coli. Purification and role in the replication of phiX174 DNA.
    J Biol Chem. 1978 Jan 10;253(1):261-9 PMID: 145439
  11. The RNA primer synthesized by primase to initiate phage G4 DNA replication.
    J Biol Chem. 1978 Feb 10;253(3):765-9 PMID: 340458
  12. A ribo-deoxyribonucleotide primer synthesized by primase.
    J Biol Chem. 1978 Feb 10;253(3):770-4 PMID: 340459
  13. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  14. An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):645-9 PMID: 204927
  15. ATP utilization by rep protein in the catalytic separation of DNA strands at a replicating fork.
    J Biol Chem. 1978 May 10;253(9):3298-304 PMID: 147874
  16. A multienzyme system for priming the replication of phiX174 viral DNA.
    J Biol Chem. 1978 May 10;253(9):3313-9 PMID: 346590
  17. The dnaB gene product of Escherichia coli. I. Purification, homogeneity, and physical properties.
    J Biol Chem. 1978 Jun 10;253(11):4043-50 PMID: 206559
  18. The dnaB gene product of Escherichia coli. II. Single stranded DNA-dependent ribonucleoside triphosphatase activity.
    J Biol Chem. 1978 Jun 10;253(11):4051-7 PMID: 206560
  19. Nucleotide sequences of the separate origins of synthesis of bacteriophage G4 viral and complementary DNA strands.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1081-5 PMID: 274698
  20. Site-specific initiation of a DNA fragment: nucleotide sequence of the bacteriophage G4 negative-strand initiation site.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3094-8 PMID: 277911
  21. The role of guanosine 5'-triphosphate in polypeptide chain elongation.
    Biochim Biophys Acta. 1978 Sep 21;505(1):95-127 PMID: 361078
  22. Energy coupling in DNA gyrase and the mechanism of action of novobiocin.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4838-42 PMID: 368801
  23. Aspects of DNA replication.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:1-9 PMID: 289448
  24. Enzymatic conversion of single-stranded phiX174 and G4 circles to duplex forms: discontinuous replication.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:289-93 PMID: 225101
  25. Probing DNA replication mechanisms with the T4 bacteriophage in vitro system.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:469-87 PMID: 225114
  26. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  27. A general priming system employing only dnaB protein and primase for DNA replication.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4308-12 PMID: 228295
  28. Studies on 30S ribosomal protein S1 from E. coli. I. Purification and physicochemical properties.
    J Biochem. 1979 Dec;86(6):1725-37 PMID: 393700
  29. Replication of the colicin E1 plasmid in extracts of Escherichia coli: uncoupling of leading strand from lagging strand synthesis.
    Mol Gen Genet. 1979;177(1):113-20 PMID: 231724
  30. An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):799-803 PMID: 6444725
  31. Initiation of DNA replication by the Escherichia coli dnaG protein: evidence that tertiary structure is involved.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):900-4 PMID: 6244591
  32. A prepriming DNA replication enzyme of Escherichia coli. I. Purification of protein n': a sequence-specific, DNA-dependent ATPase.
    J Biol Chem. 1980 Jul 25;255(14):6789-93 PMID: 6104665
  33. Replication of duplex DNA of phage phi X174 reconstituted with purified enzymes.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3322-6 PMID: 6447874
  34. Migration of Escherichia coli dnaB protein on the template DNA strand as a mechanism in initiating DNA replication.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4190-4 PMID: 144914
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.2.707
Published
1981-02-00
Pages
707-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319871
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.2.707 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2