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PMID: 6262717 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence organisation in nuclear DNA from Physarum polycephalum: methylation of repetitive sequences.

Nucleic acids research ·Vol. 9 ·No. 4 ·1981-02-25 ·Pages 801-14

Whittaker PA, McLachlan A, Hardman N

Abstract

Nuclear DNA from the slime mould Physarum polycephalum is digested by the restriction endonuclease HpaII to generate a high molecular weight and a low molecular weight component. These are referred to as the M+ and the M- compartment, respectively. Sequences that are present in the M+ compartment are cleaved by MspI, the restriction enzyme isoschizomer of HpaII, thus showing that the recognition sequences for these enzymes in M+ DNA contain methylated CpG doublets. The distribution of repetitive sequences in the M+ and M- DNA compartments was investigated by comparison of the 'fingerprint' patterns of total Physarum DNA and isolated M+ DNA after digestion using different restriction endonucleases, and by probing for the presence of specific repetitive sequences in Southern blots of M+ and M- DNA by the use of cloned DNA segments. Both types of experiment indicate that many repetitive sequences are shared by both compartments, though some repetitive sequences appear to be considerably enriched, or are present exclusively, either in M+ DNA or in M- DNA.

MeSH Terms
Base Sequence Cell Nucleus/analysis Centrifugation, Density Gradient DNA/analysis DNA Restriction Enzymes/metabolism Methylation Molecular Weight Nucleic Acid Hybridization Physarum/genetics Repetitive Sequences, Nucleic Acid/drug effects
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Whittaker P A
McLachlan A
Hardman N
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.4.801
Published
1981-02-25
Pages
801-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326713
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.4.801 PMC Full Text View journal details
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