A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway.

Nucleic acids research ·Vol. 8 ·No. 14 ·1980-07-25 ·Pages 3125-31

Makula RA, Meagher RB

Abstract

The purification and characterization of a new restriction endonuclease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence (see formula index) and cleaves at the position indicated by the arrows. The enzyme preparation obtained is suitable for restriction mapping an ligation.

MeSH Terms

Base Sequence DNA Restriction Enzymes/isolation & purification,metabolism DNA, Viral/metabolism Deoxyribonucleases, Type II Site-Specific Desulfovibrio/enzymology Electrophoresis, Polyacrylamide Gel

Chemicals

DNA, Viral DNA Restriction Enzymes endodeoxyribonuclease DdeI Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Makula R A

Meagher R B

References (6)

6 references, click to expand

Specificity of substrate recognition by the EcoRI restriction endonuclease.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310-4 PMID: 242001
A general method for the purification of restriction enzymes.
Nucleic Acids Res. 1978 Jul;5(7):2373-80 PMID: 673857
A specific endonuclease from Bacillus caldolyticus.
Nucleic Acids Res. 1978 Oct;5(10):3457-67 PMID: 724493
A computer assisted method for the determination of restriction enzyme recognifion sites.
Nucleic Acids Res. 1978 Nov;5(11):4105-27 PMID: 724510
Site specific endonuclease from Fusobacterium nucleatum.
Nucleic Acids Res. 1979 Jan;6(1):1-15 PMID: 424284
A restriction enzyme from Fusobacterium nucleatum 4H which recognizes GCNGC.
Nucleic Acids Res. 1979 Jan;6(1):17-25 PMID: 424288

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.14.3125

Published

1980-07-25

Pages

3125-31

Language

English

Region

England

NLM ID

0411011

PMCID

PMC324359

Subset

External Links

PubMed source DOI: 10.1093/nar/8.14.3125 PMC Full Text View journal details

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A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway.

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