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PMID: 6254733 Published · ppublish English Journal Article

Isolation and characterization of a new family of mobile dispersed genetic elements, mdg3, in Drosophila melanogaster.

Chromosoma ·Vol. 81 ·No. 1 ·1980-00-00 ·Pages 27-53

Ilyin YV, Chmeliauskaite VG, Ananiev EV, Georgiev GP

Abstract

The properties of a new family of mobile dispersed genetic elements, mdg3, are described. Mdg3 is represented by a DNA fragment of about 5.5 kb long which is framed with two repetitive sequences 300-500 base pairs in length. Virtually the whole region of mdg3 including the repetitive sequences is transcribed and, as the result, 26S poly(A)+RNA is formed. The repetitive sequence is present at least at the 3'-end of a major 26S transcript. Both strands of mdg3 are transcribed, but one direction is predominant. Besides 26S RNA, a minor transcript, 15S poly(A)+RNA, can be observed. 15S RNA dose not contain sequences corresponding to the middle of mdg3. Possibly it is formed from 26S RNA by means of splicing. About 15 copies of mdg3 are present in the genome of embryonic cells and polytene chromosomes. Different copies of mdg3 are identical but surrounded with different DNA sequences. In culture cells, the gene is amplified and the number of copies is increased approximately 13 times. Mdg3 is localized in 15-17 sites on different chromosomes of D. melanogaster. Its location varies from one animal to another. The properties and possible nature of mobile dispersed genetic elements are discussed.

MeSH Terms
Animals Base Sequence Chromosome Mapping DNA/analysis DNA Replication DNA Restriction Enzymes/metabolism DNA Transposable Elements Drosophila melanogaster/genetics Genes Nucleic Acid Hybridization RNA, Ribosomal/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ilyin Y V
Chmeliauskaite V G
Ananiev E V
Georgiev G P
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32 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00292421
Published
1980-00-00
Pages
27-53
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00292421 View journal details
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