A relationship between plasmid structure, structural lability, and sensitivity to site-specific endonucleases in Neisseria gonorrhoeae.

Molecular & general genetics : MGG ·Vol. 177 ·No. 2 ·1980-01-00 ·Pages 251-60

Davies JK, Normark S

Abstract

Nearly all gonococcal strains carry a small "phenotypically cryptic" plasmid of approximately 4,200 basepairs. A detailed physical map of this plasmid has been constructed, revealing the presence of numerous putative inverted repeats. These studies also revealed the presence on the plasmid of recognition sequences for several site-specific endonucleases (particularly HpaII, MspI and AluI) that are particularly resistant to cleavage, and confirmed previous reports of structural lability. Both the sites that are resistant to cleavage, and the observed structural variation are associated with the inverted repetitive sequences.

MeSH Terms

Base Composition Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA, Bacterial/genetics Genetic Variation Neisseria gonorrhoeae/genetics Plasmids Structure-Activity Relationship Substrate Specificity

Chemicals

DNA, Bacterial DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Davies J K

Normark S

References (16)

16 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267436

Published

1980-01-00

Pages

251-60

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267436

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A relationship between plasmid structure, structural lability, and sensitivity to site-specific endonucleases in Neisseria gonorrhoeae.

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