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PMID: 1088825 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of region preceding trp mRNA initiation site and its role in promoter and operator function.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2351-5

Bennett GN, Schweingruber ME, Brown KD, Squires C, Yanofsky C

Abstract

The nucleotide sequence of the region preceding the transcription initiation site of the tryptophan operon of Escherichia coli was determined. Essentially all of the trp operator precedes the transcribed portion of the operon. The deduced sequence contains the recognition site of endonuclease Hpa I. This site is protected from Hpa I cleavage by RNA polymerase and by trp repressor. Regions of 2-fold symmetry are present in the DNA sequence.

MeSH Terms
Base Sequence Coliphages/metabolism DNA, Viral/analysis,metabolism Genes, Regulator Nucleic Acid Hybridization Operon RNA, Viral/analysis Transcription, Genetic Tryptophan
Chemicals
DNA, Viral RNA, Viral Tryptophan
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bennett G N
Schweingruber M E
Brown K D
Squires C
Yanofsky C
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2351
Published
1976-07-00
Pages
2351-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430561
Subset
IM
Databases
GENBANK
J01714, M12471, M12472
External Links
PubMed source DOI: 10.1073/pnas.73.7.2351 PMC Full Text View journal details
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