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PMID: 6194991 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Vectors containing a prokaryotic dihydrofolate reductase gene transform Drosophila cells to methotrexate-resistance.

The EMBO journal ·Vol. 2 ·No. 7 ·1983-00-00 ·Pages 1099-104

Bourouis M, Jarry B

Abstract

Transformed Drosophila Kc cell lines, resistant to methotrexate, an inhibitor of de novo purine and pyrimidine synthesis, have been obtained by calcium phosphate transfection of plasmids containing a sequence coding for a methotrexate-resistant dihydrofolate reductase enzyme (DHFR). The introduced DNA is stably maintained in the cells as head-to-tail multimeric structures of the initial DNA sequence even after several months of culture in the presence of the selective agent. The introduced sequences are present at a high copy number in the transformed cells and express cytoplasmic RNAs transcribed from the DHFR gene.

MeSH Terms

Animals Cell Line DNA/isolation & purification Drosophila/genetics Drug Resistance Gene Amplification Genetic Vectors Methotrexate/pharmacology Nucleic Acid Conformation Plasmids RNA/isolation & purification Tetrahydrofolate Dehydrogenase/genetics Transformation, Genetic

Chemicals

RNA DNA Tetrahydrofolate Dehydrogenase Methotrexate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bourouis M

Jarry B

References (29)

29 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1983-00-00

Pages

1099-104

Language

English

Region

England

NLM ID

8208664

PMCID

PMC555241

Subset

External Links

PubMed source PMC Full Text View journal details

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Vectors containing a prokaryotic dihydrofolate reductase gene transform Drosophila cells to methotrexate-resistance.

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