The 5' termini of RNAs encoded by the transposable element copia.

Nucleic acids research ·Vol. 9 ·No. 23 ·1981-12-11 ·Pages 6279-91

Flavell AJ, Levis R, Simon MA, Rubin GM

Abstract

The 5' termini of copia-specific RNAs in Drosophila melanogaster tissue culture cells were determined by S1 nuclease mapping and cap analysis. Both major copia RNAs share an identical set of heterogeneous 5' ends. Three major cap 1 structures M7GpppCmpUp, M7GpppCmpCp and M7GpppGmpUp together with several other minor caps were found. Almost all the 5' termini, as judged by S-1 nuclease mapping, were located either in a pyrimidine-rich part of the terminal direct repeat or apparently outside of the copia element, suggesting that a proportion of copia transcripts derive from promoters external to the genetic element.

MeSH Terms

Animals Base Sequence DNA/genetics DNA Restriction Enzymes Drosophila melanogaster/genetics Nucleic Acid Hybridization Operon RNA Caps/genetics Transcription, Genetic

Chemicals

RNA Caps DNA DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Flavell A J

Levis R

Simon M A

Rubin G M

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32 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.23.6279

Published

1981-12-11

Pages

6279-91

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327603

Subset

Databases

GENBANK

J01075

External Links

PubMed source DOI: 10.1093/nar/9.23.6279 PMC Full Text View journal details

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The 5' termini of RNAs encoded by the transposable element copia.

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