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PMID: 6194509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential inhibition of cellular RNAs by photosensitized trioxalen.

Nucleic acids research ·Vol. 11 ·No. 18 ·1983-09-24 ·Pages 6453-64

Shames B, Kalma S, Heimer YM, Tal J

Abstract

Treatment of mouse cell cultures with 5 X 10(-6)M trioxalen (4,5',8-trimethylpsoralen, or TMP) followed by long wavelength ("near") UV-irradiation (NUV) resulted in immediate cessation of most of the cellular transcriptional activity. The effective inhibitory TMP concentrations for ribosomal RNA were lower than those affecting poly RNA containing RNA. Low molecular RNA species were only partially inhibited. Side effects, commonly caused by other drugs, were not detected. These properties, and the simplicity of their use, make psoralens attractive inhibitors of RNA biosynthesis.

MeSH Terms
Animals Cell Survival/drug effects,radiation effects DNA Replication/drug effects,radiation effects Furocoumarins/pharmacology Kinetics L Cells/drug effects,metabolism,radiation effects Mice RNA/genetics,radiation effects Transcription, Genetic/drug effects,radiation effects Trioxsalen/pharmacology Ultraviolet Rays
Chemicals
Furocoumarins RNA Trioxsalen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shames B
Kalma S
Heimer Y M
Tal J
References (25)
25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.18.6453
Published
1983-09-24
Pages
6453-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326385
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.18.6453 PMC Full Text View journal details
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