Home LiteratureArticle Details
PMID: 125412 Published · ppublish English Journal Article

The cleavage of transfer RNA by a single strang specific endonuclease from Neurospora crassa.

Nucleic acids research ·Vol. 2 ·No. 7 ·1975-07-00 ·Pages 1073-82

Tal J

Abstract

Endonuclease from Neurospora crassa (NcNase), an enzyme with specificity for polynucleotides lacking an ordered structure, was shown to cleave su+3 tRNATyr from E. coli preferentially in the anticodon region. The enzyme cleaved unfractionated tRNA primarily to 30 - 50 nucleotide size fragments, implying that most or all tRNA species are also cleaved in the anticodon region. The 3' terminal sequence C-A was cleaved as well. The results are discussed with respect to the three dimensional structure of tRNA.

MeSH Terms
Anticodon Autoradiography Coliphages/enzymology Electrophoresis, Paper Electrophoresis, Polyacrylamide Gel Endonucleases Escherichia coli Kinetics Neurospora/enzymology Neurospora crassa/enzymology Nucleic Acid Conformation Oligonucleotides/isolation & purification Polynucleotides/isolation & purification RNA, Transfer Ribonucleases
Chemicals
Anticodon Oligonucleotides Polynucleotides RNA, Transfer Endonucleases Ribonucleases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Tal J
References (22)
22 references, click to expand
  1. A simplified method for study of RNA conformation--reaction of formylmethionine transfer RNA with [14C]methylamine-bisulfite.
    Nucleic Acids Res. 1974 Oct;1(10):1305-16 PMID: 10793691
  2. Correlation between three-dimensional structure and chemical reactivity of transfer RNA.
    Nucleic Acids Res. 1974 Jul;1(7):927-32 PMID: 10793725
  3. Incorporation of terminal ribonucleotides into soluble ribonucleic acid by a purified rat liver enzyme.
    J Biol Chem. 1963 Jun;238:2102-12 PMID: 14024923
  4. AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. II. STUDIES OF ENZYME SPECIFICITY.
    J Biol Chem. 1965 Mar;240:1294-304 PMID: 14284739
  5. SPECIFIC CLEAVAGE OF THE YEAST ALANINE RNA INTO TWO LARGE FRAGMENTS.
    Proc Natl Acad Sci U S A. 1965 Mar;53:543-8 PMID: 14338233
  6. The ribonuclease activities of the single-strand-specific nucleases of Neurospora crassa.
    Can J Biochem. 1973 May;51(5):569-80 PMID: 4267583
  7. The nucleotide sequences of tyrosine transfer RNAs of Escherichia coli.
    Eur J Biochem. 1970 Apr;13(3):461-83 PMID: 4315419
  8. Action of venom phosphodiesterase on transfer RNA from Escherichia coli.
    Biochim Biophys Acta. 1970 Sep 17;217(1):176-88 PMID: 4323577
  9. Binding of complementary oligonucleotides to free and aminoacyl transfer ribonucleic acid synthetase bound transfer ribonucleic acid.
    Biochemistry. 1972 Feb 15;11(4):642-6 PMID: 4334910
  10. Three-dimensional structure of tRNA.
    Prog Nucleic Acid Res Mol Biol. 1971;11:391-421 PMID: 4339145
  11. Partial degradation of transfer RNAs and transfer RNA fragments by spleen phosphodiesterase as studied by disc electrophoretic methods.
    Biochim Biophys Acta. 1972 Sep 14;277(3):523-38 PMID: 4341777
  12. Biological activity of Escherichia coli tRNA Phe modified in its C-C-A terminus.
    Eur J Biochem. 1972 Aug 4;28(4):478-91 PMID: 4343082
  13. Specific hybridization of tyrosine transfer ribonucleic acids with DNA from a transducing bacteriophage phi-80 carrying the amber suppressor gene su 3.
    J Mol Biol. 1967 Nov 14;29(3):457-71 PMID: 4867493
  14. On the conformation of transfer RNA.
    Proc Natl Acad Sci U S A. 1968 Dec;61(4):1384-91 PMID: 4884685
  15. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.
    Biochem J. 1969 Jun;113(1):131-8 PMID: 4896715
  16. More mutant tyrosine transfer ribonucleic acids.
    J Mol Biol. 1970 Nov 28;54(1):1-14 PMID: 4923867
  17. A two-dimensional fractionation procedure for radioactive nucleotides.
    J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
  18. Complementary oligonucleotide binding to the anticodon loop of fMet-transfer RNA.
    Nature. 1970 Feb 7;225(5232):508-10 PMID: 5411856
  19. Partial nuclease digestion of transfer ribonucleic acids and aminoacylated transfer ribonucleic acids.
    Eur J Biochem. 1971 Feb;18(4):496-502 PMID: 5545006
  20. Chemical studies on amino acid acceptor ribonucleic acids. VII. Partial ribonuclease digestion of alanine and valine soluble ribonucleic acid from yeast.
    Biochemistry. 1966 Sep;5(9):3027-36 PMID: 5961891
  21. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
    Biochemistry. 1967 Jun;6(6):1818-27 PMID: 6035921
  22. Chemical modification of yeast alanine-tRNA with a radioactive carbodiimide.
    Science. 1967 Nov 3;158(3801):666-9 PMID: 6058001
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/2.7.1073
Published
1975-07-00
Pages
1073-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343494
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/2.7.1073 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2