Specific cleavage of tRNA by nuclease S1.

Nucleic acids research ·Vol. 2 ·No. 6 ·1975-06-00 ·Pages 865-71

Harada F, Dahlberg JE

Abstract

Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for rapid identification of an anticodon-containing oligonucleotide and subsequent identification of the probable amino acid specificity of tRNA.

MeSH Terms

Animals Anticodon Autoradiography Base Sequence Chick Embryo Chromatography, Paper Chromatography, Thin Layer Escherichia coli Fibroblasts Oligonucleotides/isolation & purification RNA, Transfer/metabolism Ribonucleases

Chemicals

Anticodon Oligonucleotides RNA, Transfer Ribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Harada F

Dahlberg J E

References (12)

12 references, click to expand

Small RNAs of Rous sarcoma virus: characterization by two-dimensional polyacrylamide gel electrophoresis and fingerprint analysis.
J Virol. 1973 Dec;12(6):1226-37 PMID: 4357505
The nucleotide sequence of a leucine transfer RNA from E. coli.
FEBS Lett. 1970 Sep 6;9(3):168-170 PMID: 11947660
A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.
Biochim Biophys Acta. 1966 Jan 18;114(1):158-68 PMID: 4287053
Nucleotide sequence from the coat protein cistron of R17 bacteriophage RNA.
Nature. 1969 Sep 6;223(5210):1009-14 PMID: 5811898
A two-dimensional fractionation procedure for radioactive nucleotides.
J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
Structure of yeast phenylalanine tRNA at 3 A resolution.
Nature. 1974 Aug 16;250(467):546-51 PMID: 4602655
Minor components in transfer RNA: their characterization, location, and function.
Prog Nucleic Acid Res Mol Biol. 1972;12:49-85 PMID: 4557059
Three-dimensional tertiary structure of yeast phenylalanine transfer RNA.
Science. 1974 Aug 2;185(4149):435-40 PMID: 4601792
Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.
Eur J Biochem. 1973 Feb 15;33(1):192-200 PMID: 4691350
Primary sequence of glutamic acid tRNA II from Escherichia coli.
FEBS Lett. 1972 Feb 1;20(2):239-241 PMID: 11946426
Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis.
J Biol Chem. 1973 Jul 25;248(14):5024-32 PMID: 4577761
The nucleotide sequence of a methionine tRNA which functions in protein elongation in mouse myeloma cells.
Eur J Biochem. 1975 Feb 3;51(1):283-93 PMID: 1168134

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/2.6.865

Published

1975-06-00

Pages

865-71

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343473

Subset

External Links

PubMed source DOI: 10.1093/nar/2.6.865 PMC Full Text View journal details

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Specific cleavage of tRNA by nuclease S1.

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