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PMID: 4357505 Published · ppublish English Journal Article

Small RNAs of Rous sarcoma virus: characterization by two-dimensional polyacrylamide gel electrophoresis and fingerprint analysis.

Journal of virology ·Vol. 12 ·No. 6 ·1973-12-00 ·Pages 1226-37

Sawyer RC, Dahlberg JE

Abstract

Approximately 15 to 20 different species of small (4 to 7S) RNAs have been purified by two-dimensional polyacrylamide gel electrophoresis of RNA isolated from virions of Schmidt-Ruppin D strain of Rous sarcoma virus. Each species of small RNA has been isolated free of 70S RNA; nine of them, including 5S and 7S RNAs, were also found associated with the 70S genomic RNA. Most of the 4S RNAs are present at an average of less than one copy per virion. The 4S RNAs have T1 RNase (EC 2.7.7.26) fingerprints, which are very similar to those of tRNAs. One of the smallest 4S RNAs, which can act as a primer for initiation of RNA-directed DNA synthesis, is associated with the 70S RNA in 1 to 2 copies per complex, whereas an additional 6 to 8 copies of this molecule are free.

MeSH Terms
Autoradiography Avian Sarcoma Viruses/analysis Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Methods Nucleic Acid Denaturation Oligonucleotides/analysis Phosphorus Radioisotopes RNA, Viral/analysis,isolation & purification Ribonucleases
Chemicals
Oligonucleotides Phosphorus Radioisotopes RNA, Viral Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sawyer R C
Dahlberg J E
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30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.6.1226-1237.1973
Published
1973-12-00
Pages
1226-37
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356763
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.6.1226-1237.1973 PMC Full Text View journal details
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