Home LiteratureArticle Details
PMID: 6193096 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Promoter for the unc operon of Escherichia coli.

Journal of bacteriology ·Vol. 155 ·No. 3 ·1983-09-00 ·Pages 1271-8

Porter AC, Brusilow WS, Simoni RD

Abstract

Fragments of DNA carrying possible promoters for the unc operon of Escherichia coli were cloned into a promoter detection plasmid (pRZ5255). Similar fragments were transcribed in vitro to produce transcripts whose sizes were used to determine the approximate start site for transcription. One strong promoter and at least two very much weaker ones were detected by these methods. The exact position of the strongest promoter, presumed to be the true unc promoter, was determined by S1 nuclease mapping and shown to lie 73 base pairs upstream from the open reading frame that precedes uncB. It therefore appears that this reading frame (uncI) is part of the unc operon. S1 mapping also revealed the presence of a third weak promoter 25 base pairs upstream of uncI. All of the weak promoters occur between the proposed unc promoter and uncB, but their role in vivo, if any, is unclear.

MeSH Terms
Adenosine Triphosphatases/genetics Cloning, Molecular Endonucleases Escherichia coli/enzymology,genetics Genes, Bacterial Operon Plasmids Proton-Translocating ATPases RNA, Bacterial/analysis RNA, Messenger/analysis Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
RNA, Bacterial RNA, Messenger Endonucleases Single-Strand Specific DNA and RNA Endonucleases Adenosine Triphosphatases Proton-Translocating ATPases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Porter A C
Brusilow W S
Simoni R D
References (31)
31 references, click to expand
  1. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  2. Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4365-9 PMID: 337297
  3. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  4. Mu-induced polarity in the unc operon of Escherichia coli.
    J Bacteriol. 1978 Jun;134(3):728-36 PMID: 149112
  5. Bacteriophage lambda carrying the Escherichia coli chromosomal region of the replication origin.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):5099-103 PMID: 368808
  6. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  7. Specialized transducing phage lambda carrying the genes for coupling factor of oxidative phosphorylation of Escherichia coli: increased synthesis of coupling factor on induction of prophage lambda asn.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1126-30 PMID: 155817
  8. Membrane adenosine triphosphatases of prokaryotic cells.
    Annu Rev Biochem. 1979;48:103-31 PMID: 157712
  9. Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome.
    J Bacteriol. 1979 Oct;140(1):106-13 PMID: 159284
  10. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  11. Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98% formamide or 7 M urea.
    Methods Enzymol. 1980;65(1):299-305 PMID: 6154865
  12. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  13. Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.
    Nature. 1980 May 15;285(5761):147-51 PMID: 7374764
  14. Subunits of the adenosine triphosphatase complex translated in vitro from the Escherichia coli unc operon.
    J Bacteriol. 1980 Jul;143(1):8-17 PMID: 6447144
  15. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  16. Definition of a novel promoter for the major adenovirus-associated virus mRNA.
    Cell. 1980 Nov;22(1 Pt 1):231-42 PMID: 6253077
  17. Three genes coding for subunits of the membrane sector (F0) of the Escherichia coli adenosine triphosphatase complex.
    J Bacteriol. 1981 Jan;145(1):200-10 PMID: 6450744
  18. Attenuation in the control of expression of bacterial operons.
    Nature. 1981 Feb 26;289(5800):751-8 PMID: 7007895
  19. Nucleotide sequence of genes coding for dicyclohexylcarbodiimide-binding protein and the alpha subunit of proton-translocating ATPase of Escherichia coli.
    Biochem Biophys Res Commun. 1981 May 15;100(1):219-25 PMID: 6266400
  20. The atp operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the delta subunit of Escherichia coli ATP-synthase.
    Nucleic Acids Res. 1981 Aug 25;9(16):3919-26 PMID: 6272190
  21. The atp operon: nucleotide sequence of the genes for the gamma, beta, and epsilon subunits of Escherichia coli ATP synthase.
    Nucleic Acids Res. 1981 Oct 24;9(20):5287-96 PMID: 6272217
  22. The atp operon: nucleotide sequence of the region encoding the alpha-subunit of Escherichia coli ATP-synthase.
    Nucleic Acids Res. 1981 May 11;9(9):2187-94 PMID: 6272228
  23. Nucleotide sequence of the genes coding for alpha, beta and gamma subunits of the proton-translocating ATPase of Escherichia coli.
    Biochem Biophys Res Commun. 1981 Nov 30;103(2):604-12 PMID: 6277310
  24. Nucleotide sequence of the genes for F0 components of the proton-translocating ATPase from Escherichia coli: prediction of the primary structure of F0 subunits.
    Biochem Biophys Res Commun. 1981 Nov 30;103(2):613-20 PMID: 6277311
  25. The nucleotide sequence of the atp genes coding for the F0 subunits a, b, c and the F1 subunit delta of the membrane bound ATP synthase of Escherichia coli.
    Mol Gen Genet. 1981;184(1):33-9 PMID: 6278247
  26. Gene order and gene-polypeptide relationships of the proton-translocating ATPase operon (unc) of Escherichia coli.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):320-4 PMID: 6281763
  27. Nucleotide sequence of the promoter region of the gene cluster for proton-translocating ATPase from Escherichia coli and identification of the active promotor.
    Biochem Biophys Res Commun. 1982 Jul 30;107(2):568-75 PMID: 6215041
  28. Promoter occlusion: transcription through a promoter may inhibit its activity.
    Cell. 1982 Jul;29(3):939-44 PMID: 6217898
  29. Promoters of the atp operon coding for the membrane-bound ATP synthase of Escherichia coli mapped by Tn10 insertion mutations.
    Mol Gen Genet. 1982;188(2):240-8 PMID: 6185823
  30. Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):653-7 PMID: 6187001
  31. In vivo 5' terminus and length of the mRNA for the proton-translocating ATPase (unc) operon of Escherichia coli.
    J Bacteriol. 1983 Sep;155(3):1279-87 PMID: 6193097
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.155.3.1271-1278.1983
Published
1983-09-00
Pages
1271-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217825
Subset
IM
Grants
NIGMS NIH HHS · GM07598 · United States
NIGMS NIH HHS · GM18539 · United States
External Links
PubMed source DOI: 10.1128/jb.155.3.1271-1278.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2