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PMID: 6272228 Published · ppublish English Journal Article

The atp operon: nucleotide sequence of the region encoding the alpha-subunit of Escherichia coli ATP-synthase.

Nucleic acids research ·Vol. 9 ·No. 9 ·1981-05-11 ·Pages 2187-94

Gay NJ, Walker JE

Abstract

Part of the atp (or unc) operon encoding the alpha, beta, gamma, delta, and epsilon subunits of Escherichia coli ATP-synthase has been cloned into the plasmid pACYC 184. The DNA coding for the largest of these proteins, the alphas subunit, has been sequenced by cloning into the bacteriophage M13 and sequencing with dideoxy nucleotide chain terminators. It comprises 1539 nucleotides corresponding to a protein of 513 amino acids.

MeSH Terms
ATP Synthetase Complexes Adenosine Diphosphate/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific Escherichia coli/enzymology Multienzyme Complexes/genetics Operon Phosphotransferases/genetics Plasmids
Chemicals
DNA, Bacterial Multienzyme Complexes Adenosine Diphosphate Phosphotransferases ATP Synthetase Complexes DNA Restriction Enzymes Deoxyribonuclease EcoRI endodeoxyribonuclease HpaI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gay N J
Walker J E
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.9.2187
Published
1981-05-11
Pages
2187-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326834
Subset
IM
Databases
GENBANK
J01594, J01595, K02181, V00264, V00313, X00771
External Links
PubMed source DOI: 10.1093/nar/9.9.2187 PMC Full Text View journal details
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