Home LiteratureArticle Details
PMID: 6190071 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A new gene in E. coli RNA synthesis.

Molecular & general genetics : MGG ·Vol. 189 ·No. 2 ·1983-00-00 ·Pages 314-20

Liebke HH, Speyer JF

Abstract

A novel spontaneous temperature sensitive mutant of Escherichia coli, which stops synthesizing stable RNA and some proteins immediately upon temperature shift from 30 degrees C to 42 degrees C, is described. Stable RNA species are not preferentially degraded in the mutant at the nonpermissive temperature. The guanine polyphosphate compounds, ppGpp (MS1) and pppGpp (MS2), are not produced at 42 degrees C. The mutant strain does not grow at 42 degrees C in either broth or defined minimal medium supplemented with any of a variety of carbon sources. The temperature sensitive mutation in this strain maps between dap A, E and pts I and defines a new locus affecting RNA synthesis in E. coli.

MeSH Terms
Escherichia coli/genetics,metabolism Genes, Bacterial Guanine Nucleotides/biosynthesis Mutation RNA, Bacterial/biosynthesis,genetics Temperature
Chemicals
Guanine Nucleotides RNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Liebke H H
Speyer J F
References (49)
49 references, click to expand
  1. In vivo transcription of rRNA operons in Escherichia coli initiates with purine nucleoside triphosphates at the first promoter and with CTP at the second promoter.
    J Biol Chem. 1979 Jul 10;254(13):5609-12 PMID: 376515
  2. In vitro transcripts from the rrn B ribosomal RNA cistron originate from two tandem promoters.
    Cell. 1979 Jan;16(1):111-21 PMID: 369703
  3. Chloramphenicol-induced changes in the synthesis of ribosomal, transfer, and messenger ribonucleic acids in Escherichia coli B/r.
    J Bacteriol. 1977 Jun;130(3):1098-108 PMID: 324974
  4. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5 PMID: 368799
  5. Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger-RNA.
    Proc Natl Acad Sci U S A. 1962 Apr 15;48:532-46 PMID: 13877975
  6. Regulation of bacterial ppGpp and pppGpp.
    Annu Rev Microbiol. 1975;29:301-18 PMID: 170852
  7. T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease 3.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3296-3300 PMID: 4587248
  8. Transient rates of synthesis of individual polypeptides in E. coli following temperature shifts.
    Cell. 1978 Mar;13(3):427-34 PMID: 350413
  9. Identification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):5036-40 PMID: 1108010
  10. Regulation of ribonucleic acid synthesis in growing bacterial cells. I. Control over the total rate of RNA synthesis.
    J Mol Biol. 1972 Dec 30;72(3):751-64 PMID: 4573847
  11. A rapid test for the rel A mutation in E. coli.
    Biochem Biophys Res Commun. 1976 Apr 5;69(3):751-8 PMID: 773380
  12. A mutation in the RNA polymerase beta' subunit causing depressed ribosomal RNA synthesis in Escherichia coli.
    Mol Gen Genet. 1981;183(1):54-8 PMID: 6799741
  13. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5.
    Genetics. 1979 Jul;92(3):741-7 PMID: 395018
  14. Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase.
    Mol Gen Genet. 1974;132(3):193-201 PMID: 4607989
  15. Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli.
    Cell. 1978 Nov;15(3):1055-66 PMID: 365352
  16. Partial purification and properties of a ribosomal RNA maturation endonuclease from Bacillus subtilis.
    J Biol Chem. 1977 Feb 25;252(4):1350-7 PMID: 402365
  17. A conditional lethal mutant of Escherichia coli which affects the processing of ribosomal RNA.
    J Biol Chem. 1978 Mar 10;253(5):1738-42 PMID: 342528
  18. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  19. Deletion analysis of the expression of rRNA genes and associated tRNA genes carried by a lambda transducing bacteriophage.
    J Bacteriol. 1979 Jan;137(1):507-16 PMID: 368028
  20. Temperature-induced synthesis of specific proteins in Escherichia coli: evidence for transcriptional control.
    J Bacteriol. 1980 Jun;142(3):843-51 PMID: 6155374
  21. Properties of a mutant of Escherichia coli defective in bacteriophage lambda head formation (groE). I. Initial characterization.
    J Mol Biol. 1973 May 5;76(1):1-23 PMID: 4578098
  22. Genetics of bacterial ribosomes.
    Annu Rev Genet. 1977;11:297-347 PMID: 339818
  23. Insertions of Tn 10 into an E. coli ribosomal RNA operon are incompletely polar.
    Cell. 1980 Aug;21(1):257-65 PMID: 6157478
  24. A genetic locus for the regulation of ribonucleic acid synthesis.
    Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005-14 PMID: 13916843
  25. Synthesis of ribosomal RNA on a protein-DNA complex isolated from bacteria: a comparison of ribosomal RNA synthesis in vitro and in vivo.
    J Mol Biol. 1970 Sep 14;52(2):281-300 PMID: 4991980
  26. Initiation of Escherichia coli ribosomal RNA synthesis in vivo.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5480-4 PMID: 392503
  27. Relationship between the concentration of nucleoside triphosphates and the rate of synthesis of RNA.
    J Mol Biol. 1973 Jun 25;78(1):117-21 PMID: 4581289
  28. Rifampicin inhibition of ribonucleic acid and protein synthesis in normal and ethylenediaminetetraacetic acid-treated Escherichia coli.
    J Bacteriol. 1970 Oct;104(1):376-89 PMID: 4990763
  29. Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli.
    J Bacteriol. 1977 Dec;132(3):931-49 PMID: 336613
  30. Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1831-5 PMID: 325555
  31. Regulation of ribosome production in Escherichia coli: synthesis and stability of ribosomal RNA and of ribosomal protein messenger RNA at different growth rates.
    J Mol Biol. 1977 Sep 25;115(3):335-54 PMID: 338910
  32. The temperature sensitive mutant 72c. I. Pleiotropic growth behaviour and changed response to some antibiotics and mutations in the transcription or translation apparatus.
    Mol Gen Genet. 1978 Apr 25;161(1):9-14 PMID: 353503
  33. Tandem promoters direct E. coli ribosomal RNA synthesis.
    Cell. 1979 May;17(1):225-34 PMID: 110460
  34. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  35. Regulation of bacterial growth, RNA, and protein synthesis.
    Annu Rev Microbiol. 1978;32:393-432 PMID: 360971
  36. Isolation of a Mutant of Escherichia coli with a Temperature-sensitive Fructose-1,6-Diphosphate Aldolase Activity.
    J Bacteriol. 1966 Aug;92(2):464-9 PMID: 16562136
  37. Altered chemical properties in three mutants of E. coli RNA polymerase sigma subunit.
    Mol Gen Genet. 1979 Oct 1;175(3):251-7 PMID: 392226
  38. Genetic mapping of a putative temperature-sensitive transcription mutation in Escherichia coli K12.
    Mol Gen Genet. 1978 Oct 30;166(2):211-6 PMID: 370546
  39. Titration of the gene sites on DNA by DNA-RNA hybridization. II. The Escherichia coli chromosome.
    J Mol Biol. 1968 May 28;34(1):85-103 PMID: 4938545
  40. Serine sensitivity of Escherichia coli K 12: partial characterization of a serine resistnat mutant that is extremely sensitive to 2-ketobutyrate.
    Mol Gen Genet. 1980 Apr;178(1):155-64 PMID: 6247616
  41. A procedure for isolation of spontaneous mutants with temperature sensitive of RNA and/or protein.
    Mol Gen Genet. 1977 Nov 18;156(3):233-7 PMID: 340905
  42. Noncoordinate control of RNA, lipopolysaccharide, and phospholipid syntheses during amino acid starvation in stringent and relaxed strains of Escherichia coli.
    J Bacteriol. 1978 Jul;135(1):274-7 PMID: 353033
  43. Culture medium for enterobacteria.
    J Bacteriol. 1974 Sep;119(3):736-47 PMID: 4604283
  44. The 30 S ribosomal precursor RNA from Escherichia coli. A primary transcript containing 23 S, 16 S, and 5 S sequences.
    J Biol Chem. 1975 Jul 25;250(14):5647-54 PMID: 1095585
  45. Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli.
    Cell. 1977 Aug;11(4):779-93 PMID: 329999
  46. Role of rel gene in translation during amino acid starvation in Escherichia coli.
    Nature. 1974 May 3;249(452):59-62 PMID: 4598028
  47. A new mutation affecting ribosomal RNA synthesis in Escherichia coli.
    Biochemistry. 1977 Aug 9;16(16):3603-7 PMID: 407931
  48. Strain of Escherichia coli with a temperature-sensitive mutation affecting ribosomal ribonucleic acid accumulation.
    J Bacteriol. 1975 Mar;121(3):923-32 PMID: 1090609
  49. Regulation of ribonucleic acid synthesis in growing bacterial cells. II. Control over the composition of the newly made RNA.
    J Mol Biol. 1972 Dec 30;72(3):765-77 PMID: 4573848
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337823
Published
1983-00-00
Pages
314-20
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NCI NIH HHS · 1 F22 CAO2727 · United States
NIGMS NIH HHS · GM-15697 · United States
External Links
PubMed source DOI: 10.1007/BF00337823
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2