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PMID: 6168771 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation-defective mutants of Snyder-Theilen feline sarcoma virus lack tyrosine-specific protein kinase activity.

Journal of virology ·Vol. 39 ·No. 1 ·1981-07-00 ·Pages 246-54

Barbacid M, Donner L, Ruscetti SK, Sherr CJ

Abstract

Four phenotypically normal mink cell clones, each containing a transformation-defective provirus of the Snyder-Theilen strain of feline sarcoma virus (ST-FeSV), synthesized an 85,000-dalton viral polyprotein (P85) indistinguishable in size and antigenic complexity from that encoded by wild-type transforming ST-FeSV. An additional transformation-defective, ST-FeSV-containing flat cell clone produced a polyprotein of 88,000 daltons (P88). The viral polyproteins immunoprecipitated from cytoplasmic extracts of these cells lacked the tyrosine-specific protein kinase activity associated with the wild-type ST-FeSV gene product. In addition, the products encoded by representative transformation-defective ST-FeSV genomes were poorly phosphorylated in vivo and lacked detectable phosphotyrosine residues. Whereas proteins of ST-FeSV transformants contained elevated levels of phosphotyrosine, those of mink cells containing transformation-defective ST-FeSV exhibited phosphotyrosine levels no higher than those found in uninfected cells. These findings provide genetic evidence that the tyrosine-specific protein kinase activity associated with ST-FeSV P85 is required for virus-induced transformation.

MeSH Terms
Animals Cell Line Cell Transformation, Viral Genes, Viral Mink Mutation Phosphorylation Phosphotyrosine Protein Kinases/metabolism Protein-Tyrosine Kinases Retroviridae/genetics Sarcoma Viruses, Feline/enzymology,genetics Tyrosine/analogs & derivatives,metabolism Viral Proteins/genetics
Chemicals
Viral Proteins Phosphotyrosine Tyrosine Protein Kinases Protein-Tyrosine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Barbacid M
Donner L
Ruscetti S K
Sherr C J
References (28)
28 references, click to expand
  1. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  2. Transformation-defective virus mutants in a class of morphologic revertants of sarcoma virus transformed nonproducer cells.
    Cell. 1974 Aug;2(4):279-86 PMID: 4370517
  3. Thyrotrophin-releasing hormone and shaking behaviour in rat.
    Nature. 1975 Feb 27;253(5494):739-40 PMID: 803644
  4. Different mechanisms for morphologic reversion of a clonal population of murine sarcoma virus-transformed nonproducer cells.
    Virology. 1977 Apr;77(2):750-61 PMID: 193260
  5. Feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins.
    J Virol. 1977 Sep;23(3):599-607 PMID: 197262
  6. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  7. Pseudotypes of feline sarcoma virus contain an 85,000-dalton protein with feline oncornavirus-associated cell membrane antigen (FOCMA) activity.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1505-9 PMID: 206902
  8. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.
    Cell. 1978 Oct;15(2):561-72 PMID: 214242
  9. Biological properties and translational products of three independent isolates of feline sarcoma virus.
    Virology. 1979 Jan 15;92(1):91-107 PMID: 217170
  10. Cellular regulation of mammalian sarcoma virus expression: a gene regulation model for oncogenesis.
    Cell. 1979 Apr;16(4):875-84 PMID: 222469
  11. The nonstructural components of polyproteins encoded by replication-defective mammalian transforming retroviruses are phosphorylated and have associated protein kinase activity.
    Virology. 1980 Feb;101(1):185-97 PMID: 6243819
  12. Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine.
    Nature. 1980 Feb 28;283(5750):826-31 PMID: 6244493
  13. Biochemical and immunological characterization of polyproteins coded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen strains of feline sarcoma virus.
    J Virol. 1980 Jan;33(1):196-207 PMID: 6154148
  14. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  15. Transformation-defective mutants of feline sarcoma virus which express a product of the viral src gene.
    J Virol. 1980 Jul;35(1):129-40 PMID: 6251261
  16. Three independent isolates of feline sarcoma virus code for three distinct gag-x polyproteins.
    J Virol. 1980 Jul;35(1):259-64 PMID: 6251274
  17. Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virus.
    Cell. 1980 Jul;20(3):807-16 PMID: 6251974
  18. Abelson murine leukemia virus transformation-defective mutants with impaired P120-associated protein kinase activity.
    J Virol. 1980 Nov;36(2):374-86 PMID: 6253663
  19. A transformation-defective mutant of Abelson murine leukemia virus lacks protein kinase activity.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4993-7 PMID: 6254050
  20. Origin and functional properties of the major gene product of the Snyder-Theilen strain of feline sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5158-62 PMID: 6254060
  21. Transformation by Rous sarcoma virus: a cellular substrate for transformation-specific protein phosphorylation contains phosphotyrosine.
    Cell. 1980 Oct;21(3):821-8 PMID: 6159984
  22. Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product.
    Cell. 1980 Oct;21(3):829-36 PMID: 6254669
  23. Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus.
    Cell. 1980 Dec;22(3):757-65 PMID: 6257396
  24. A strain of Fujinami sarcoma virus which is temperature-sensitive in protein phosphorylation and cellular transformation.
    Cell. 1980 Dec;22(3):767-75 PMID: 6257397
  25. Tyrosine-specific protein kinase activity associated with p105 of avian sarcoma virus PRCII.
    Virology. 1981 Feb;109(1):223-8 PMID: 6258311
  26. Cellular transformation by subgenomic feline sarcoma virus DNA.
    J Virol. 1981 Jan;37(1):518-23 PMID: 6261000
  27. Evidence that the Abelson virus protein functions in vivo as a protein kinase that phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1552-6 PMID: 6262813
  28. The structure and protein kinase activity of proteins encoded by nonconditional mutants and back mutants in the sec gene of avian sarcoma virus.
    Virology. 1981 Jan 15;108(1):47-70 PMID: 6267776
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.39.1.246-254.1981
Published
1981-07-00
Pages
246-54
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171283
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.39.1.246-254.1981 PMC Full Text View journal details
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