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PMID: 6167564 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning and expression of the ilvGEDAY genes from Salmonella typhimurium.

Journal of bacteriology ·Vol. 147 ·No. 2 ·1981-08-00 ·Pages 452-62

Blazey DL, Kim R, Burns RO

Abstract

The ilvGEDAY genes of Salmonella typhimurium were cloned in Escherichia coli K-12 by in vitro recombination techniques. A single species of recombinant plasmid, designated pDU1, was obtained by selecting for Valr Ampr transformants of strain SK1592. pDU1 was shown to contain a 14-kilobase EcoRI partial digestion product of the S. typhimurium chromosome inserted into the EcoRI site of the pVH2124 cloning vector. The ilvGEDAY genes were found to occupy a maximum length of 7.5 kilobases. Restriction endonuclease analysis of the S. typhimurium ilv gene cluster provided another demonstration of the gene order as well as established the location of ilv Y between ilvA and ilvC. The presence of a ribosomal ribonucleic acid operon on the pDU1 insert, about 3 kilobases from the 5' end of ilvG, was shown by Southern hybridization. The expression of the ilvGEDA operon from pDU1 was found to be elevated, reflecting the increased gene dosage of the multicopy plasmid. A polarity was observed with respect to ilvEDA expression which is discussed in terms of the possible translational effects of the two internal promoter sequences, one located proximal to ilvE and the other located proximal to ilvD.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Gene Expression Regulation Isoleucine/biosynthesis Operon RNA, Bacterial/genetics RNA, Ribosomal/genetics Salmonella typhimurium/genetics,metabolism Valine/biosynthesis
Chemicals
RNA, Bacterial RNA, Ribosomal Isoleucine Valine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blazey D L
Kim R
Burns R O
References (41)
41 references, click to expand
  1. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.
    Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65 PMID: 5323016
  2. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.
    Mol Gen Genet. 1975 Dec 30;142(3):239-49 PMID: 1045010
  3. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.
    Biochem J. 1969 Jun;113(1):131-8 PMID: 4896715
  4. The metabolism of valine and isoleucine in Escherichia coli. XVII. The role of induction in the derepression of acetohydroxy acid isomeroreductase.
    Biochem Biophys Res Commun. 1969 Dec 4;37(6):902-8 PMID: 4902782
  5. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  6. Branched-chain amino-acid aminotransferase of Salmonella typhimurium. I. Crystallization and preliminary characterization.
    Biochim Biophys Acta. 1971 Jan 13;227(1):56-66 PMID: 5543594
  7. Circular DNA.
    Annu Rev Biochem. 1971;40:899-942 PMID: 4942224
  8. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  9. F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony.
    Bacteriol Rev. 1972 Dec;36(4):608-37 PMID: 4568766
  10. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  11. Growth of coliphage T7 in Salmonella typhimurium.
    J Virol. 1973 May;11(5):621-9 PMID: 4350708
  12. Molecular weight, subunit structure, and amino acid composition of the branched chain amino acid aminotransferase of Salmonella typhimurium.
    Biochemistry. 1974 May 7;13(10):2070-7 PMID: 4597070
  13. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  14. The subunit structure of alpha-acetohydroxyacid isomeroreductase from Salmonella typhimurium.
    J Biol Chem. 1975 Feb 10;250(3):877-82 PMID: 803501
  15. Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids.
    J Bacteriol. 1975 Jan;121(1):234-8 PMID: 1090570
  16. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  18. Rat insulin genes: construction of plasmids containing the coding sequences.
    Science. 1977 Jun 17;196(4296):1313-9 PMID: 325648
  19. Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli.
    J Bacteriol. 1977 Dec;132(3):931-49 PMID: 336613
  20. Some rRNA operons in E. coli have tRNA genes at their distal ends.
    Cell. 1978 Feb;13(2):335-44 PMID: 342117
  21. Physical organization of the ilvEDAC genes of Escherichia coli strain K-12.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):89-93 PMID: 343113
  22. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  23. Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes.
    Nucleic Acids Res. 1978 May;5(5):1445-64 PMID: 307230
  24. Linkage map of Salmonella typhimurium, edition V.
    Microbiol Rev. 1978 Jun;42(2):471-519 PMID: 353483
  25. Amino acid biosynthesis and its regulation.
    Annu Rev Biochem. 1978;47:532-606 PMID: 354503
  26. Cloning of the yeast ribosomal DNA repeat unit in SstI and HindIII lambda vectors using genetic and physical size selections.
    J Mol Biol. 1978 Aug 15;123(3):371-86 PMID: 357736
  27. Threonine deaminase from Salmonella typhimurium. Substrate-specific patterns of inhibition in an activator site-deficient form of the enzyme.
    J Biol Chem. 1979 Feb 25;254(4):1074-9 PMID: 368068
  28. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  29. Positive control of ilvC expression in Escherichia coli K-12; identification and mapping of regulatory gene ilvY.
    J Bacteriol. 1979 Sep;139(3):1014-20 PMID: 113381
  30. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  31. Genetic organization of the Salmonella typhimurium ilv gene cluster.
    Mol Gen Genet. 1979;177(1):1-11 PMID: 395408
  32. Purification and properties of S1 nuclease from Aspergillus.
    Methods Enzymol. 1980;65(1):248-55 PMID: 6246349
  33. Nucleotide sequence of ilvGEDA operon attenuator region of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1823-7 PMID: 6990415
  34. Multivalent translational control of transcription termination at attenuator of ilvGEDA operon of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1862-6 PMID: 6154938
  35. Gene ilvY of Salmonella typhimurium.
    J Bacteriol. 1980 Jun;142(3):1015-8 PMID: 6247331
  36. A new map location for the ilvB locus of Escherichia coli.
    Genetics. 1980 Sep;96(1):59-77 PMID: 7009323
  37. Reversible synthesis of polyribonucleotides with an enzyme from Escherichia coli.
    J Biol Chem. 1957 Jun;226(2):1077-92 PMID: 13438894
  38. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression.
    Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804-8 PMID: 13959618
  39. Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities.
    Nucleic Acids Res. 1975 Sep;2(9):1459-92 PMID: 1178526
  40. Mutants of Escherichia coli requiring methionine or vitamin B12.
    J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
  41. Enzymatic breakage and joining of deoxyribonucleic acid. VI. Further purification and properties of polynucleotide ligase from Escherichia coli infected with bacteriophage T4.
    J Biol Chem. 1968 Sep 10;243(17):4543-55 PMID: 4879167
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.2.452-462.1981
Published
1981-08-00
Pages
452-62
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216064
Subset
IM
Grants
NIGMS NIH HHS · GM07171 · United States
NIGMS NIH HHS · GM12551 · United States
External Links
PubMed source DOI: 10.1128/jb.147.2.452-462.1981 PMC Full Text View journal details
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