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PMID: 7009323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A new map location for the ilvB locus of Escherichia coli.

Genetics ·Vol. 96 ·No. 1 ·1980-09-00 ·Pages 59-77

Newman TC, Levinthal M

Abstract

We isolated, in E. coli K12, new alleles of the ilvB locus, the structural gene for acetolactate synthase isoenzyme I, and showed them to map at or near the ilvB619 site. The map position of the ilvB locus was redetermined because plasmids containing the ilvC-cya portion of the chromosome did not complement mutations at the ilvB locus. Furthermore, diploids for the ilvEDAC genes formed with these plasmids in an ilvHI background facilitated the isolation of the new ilvB alleles. The ilvB locus was remapped and found to be located at 81.5 minutes, between the uhp and dnaA loci. This location was determined by two- and three-point transductional crosses, deletion mapping and complementation with newly isolated plasmids. One of the new alleles of the ilvB gene is a mu-1 insertion. When present in the donor strain, this allele interferes with the linkage of genes flanking the mu-1 insertion, as well as the linkage of genes to either side of the mu-1 insertion.

MeSH Terms
Acetolactate Synthase/genetics Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics Genes Genetic Complementation Test Mutation Oxo-Acid-Lyases/genetics Plasmids Transduction, Genetic
Chemicals
Acetolactate Synthase Oxo-Acid-Lyases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Newman T C
Levinthal M
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22 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/96.1.59
Published
1980-09-00
Pages
59-77
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1214298
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/96.1.59 PMC Full Text View journal details
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