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PMID: 6151179 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 24 ·1984-12-00 ·Pages 7994-8

Sumikawa K, Parker I, Miledi R

Abstract

Poly(A)+ mRNAs extracted from embryonic chicken brain and from adult rat brain were fractionated on sucrose density gradients. The fractions were subsequently injected into Xenopus oocytes where the mRNA was translated. The products were processed and incorporated into the oocyte membrane where they formed functional neurotransmitter receptors and voltage-operated channels. Different mRNA fractions induced the incorporation of different transmitter receptors and voltage-operated channels into the oocyte membrane. These experiments provide a useful step towards the understanding of the structure and function of neurotransmitter receptors and channels.

MeSH Terms
Animals Brain/metabolism,physiology Chickens Female Ion Channels/physiology Kinetics Oocytes/metabolism Poly A/genetics Potassium/metabolism Protein Biosynthesis RNA, Messenger/genetics Rats Receptors, Neurotransmitter/genetics Sodium/metabolism Xenopus
Chemicals
Ion Channels RNA, Messenger Receptors, Neurotransmitter Poly A Sodium Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sumikawa K
Parker I
Miledi R
References (21)
21 references, click to expand
  1. Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
    J Physiol. 1982 Jul;328:143-70 PMID: 7131311
  2. Separate fractions of mRNA from Torpedo electric organ induce chloride channels and acetylcholine receptors in Xenopus oocytes.
    EMBO J. 1984 Oct;3(10):2291-4 PMID: 6094179
  3. Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
    Proc R Soc Lond B Biol Sci. 1982 May 22;215(1199):241-6 PMID: 6127706
  4. A calcium-dependent transient outward current in Xenopus laevis oocytes.
    Proc R Soc Lond B Biol Sci. 1982 Jul 22;215(1201):491-7 PMID: 6127718
  5. The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.
    Nucleic Acids Res. 1982 Oct 11;10(19):5809-22 PMID: 6183641
  6. Synthesis of chick brain GABA receptors by frog oocytes.
    Proc R Soc Lond B Biol Sci. 1982 Nov 22;216(1205):509-15 PMID: 6129641
  7. Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.
    Nature. 1983 Jan 20;301(5897):251-5 PMID: 6687403
  8. Structural homology of Torpedo californica acetylcholine receptor subunits.
    Nature. 1983 Apr 7;302(5908):528-32 PMID: 6188060
  9. Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71 PMID: 6572962
  10. Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):1111-5 PMID: 6573658
  11. Recording of single gamma-aminobutyrate- and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes.
    Proc R Soc Lond B Biol Sci. 1983 Jul 22;218(1213):481-4 PMID: 6136979
  12. Serotonin receptors induced by exogenous messenger RNA in Xenopus oocytes.
    Proc R Soc Lond B Biol Sci. 1983 Aug 22;219(1214):103-9 PMID: 6137826
  13. A transient calcium-dependent chloride current in the immature Xenopus oocyte.
    J Physiol. 1983 Sep;342:309-25 PMID: 6313909
  14. Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes.
    Proc R Soc Lond B Biol Sci. 1983 Nov 22;220(1218):131-40 PMID: 6140681
  15. The sodium channel from rat brain. Purification and subunit composition.
    J Biol Chem. 1984 Feb 10;259(3):1667-75 PMID: 6319405
  16. Expression of functional acetylcholine receptor from cloned cDNAs.
    Nature. 1984 Feb 16-22;307(5952):604-8 PMID: 6320016
  17. Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
    Nature. 1984 Mar 29-Apr 4;308(5958):421-4 PMID: 6323990
  18. Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes.
    EMBO J. 1982;1(11):1307-12 PMID: 6144542
  19. Glutamate and kainate receptors induced by rat brain messenger RNA in Xenopus oocytes.
    Proc R Soc Lond B Biol Sci. 1984 Apr 24;221(1223):127-43 PMID: 6145158
  20. Properties of human brain glycine receptors expressed in Xenopus oocytes.
    Proc R Soc Lond B Biol Sci. 1984 Apr 24;221(1223):235-44 PMID: 6145162
  21. Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence.
    Nature. 1982 Oct 28;299(5886):793-7 PMID: 6182472
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.24.7994
Published
1984-12-00
Pages
7994-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392280
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.24.7994 PMC Full Text View journal details
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