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PMID: 6140681 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes.

Proceedings of the Royal Society of London. Series B, Biological sciences ·Vol. 220 ·No. 1218 ·1983-11-22 ·Pages 131-40

Gundersen CB, Miledi R, Parker I

Abstract

Poly(A)+ messenger RNA (mRNA) extracted from rat brains or from cat muscles was injected into Xenopus laevis oocytes. This led to the incorporation of voltage-operated Na+ and K+ channels into the oocyte membrane. These channels are not normally present in the oocyte and presumably result from the synthesis and processing of proteins coded by the injected mRNA. Tetrodotoxin blocked the Na+ channels induced by mRNA derived from either innervated or denervated muscle.

MeSH Terms

Animals Brain/physiology Cats Electric Conductivity Female Genetic Engineering Ion Channels/physiology Membrane Potentials Muscles/physiology Oocytes/physiology Potassium/physiology RNA, Messenger/genetics Rats Sodium/physiology Tetrodotoxin/pharmacology Xenopus laevis

Chemicals

Ion Channels RNA, Messenger Tetrodotoxin Sodium Potassium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gundersen C B

Miledi R

Parker I

Article Info

Journal

Proceedings of the Royal Society of London. Series B, Biological sciences

Abbr.

Proc R Soc Lond B Biol Sci

ISSN

0950-1193

DOI

10.1098/rspb.1983.0092

Published

1983-11-22

Pages

131-40

Language

English

Region

England

NLM ID

7505889

Subset

External Links

PubMed source DOI: 10.1098/rspb.1983.0092 View journal details

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Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes.

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