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PMID: 6323990 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.

Nature ·Vol. 308 ·No. 5958 ·1984-00-00 ·Pages 421-4

Gundersen CB, Miledi R, Parker I

Abstract

Sodium channels and receptors to serotonin and kainate were 'transplanted' from human brain into frog oocytes, by isolating messenger RNA from a fetal brain, and injecting it into Xenopus laevis oocytes. The mRNA was translated by the oocyte and induced the appearance of functional receptors and channels in its membrane. This approach renders drug- and voltage-operated channels of the human brain more amenable to detailed study.

MeSH Terms

Animals Brain/metabolism Electric Stimulation Female Humans Ion Channels/metabolism Kainic Acid/metabolism Membrane Potentials/drug effects Oocytes/drug effects,physiology RNA, Messenger/genetics Receptors, Cell Surface/genetics,metabolism Receptors, Kainic Acid Receptors, Serotonin/genetics,metabolism Serotonin/metabolism,pharmacology Xenopus

Chemicals

Ion Channels RNA, Messenger Receptors, Cell Surface Receptors, Kainic Acid Receptors, Serotonin Serotonin Kainic Acid

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gundersen C B

Miledi R

Parker I

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/308421a0

Published

1984-00-00

Pages

421-4

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/308421a0 View journal details

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Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.

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