Home LiteratureArticle Details
PMID: 6098822 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A polyoma mutant that encodes small T antigen but not middle T antigen demonstrates uncoupling of cell surface and cytoskeletal changes associated with cell transformation.

Molecular and cellular biology ·Vol. 4 ·No. 12 ·1984-12-00 ·Pages 2774-83

Liang TJ, Carmichael GG, Benjamin TL

Abstract

The hr-t gene of polyoma virus encodes both the small and middle T (tumor) antigens and exerts pleiotropic effects on cells. By mutating the 3' splice site for middle T mRNA, we have constructed a virus mutant, Py808A, which fails to express middle T but encodes normal small and large T proteins. The mutant failed to induce morphological transformation or growth in soft agar, but did stimulate postconfluent growth of normal cells. Cells infected by Py808A became fully agglutinable by lectins while retaining normal actin cable architecture and normal levels of extracellular fibronectin. These properties of Py808A demonstrated the separability of structural changes at the cell surface from those in the cytoskeleton and extracellular matrix, parameters which have heretofore been linked in the action of the hr-t and other viral oncogenes.

MeSH Terms
Actins/metabolism Agglutination Aminoisobutyric Acids/metabolism Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Base Sequence Cell Transformation, Viral Deoxyglucose/metabolism Endonucleases/metabolism Fibronectins/metabolism Lectins/pharmacology Mice Mutation Polyomavirus/genetics,metabolism RNA, Messenger/analysis Single-Strand Specific DNA and RNA Endonucleases Viral Proteins/genetics
Chemicals
Actins Aminoisobutyric Acids Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Fibronectins Lectins RNA, Messenger Viral Proteins 2-aminoisobutyric acid Deoxyglucose Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liang T J
Carmichael G G
Benjamin T L
References (68)
68 references, click to expand
  1. Construction and expression of a recombinant DNA gene encoding a polyomavirus middle-size tumor antigen with the carboxyl terminus of the vesicular stomatitis virus glycoprotein G.
    Mol Cell Biol. 1984 Feb;4(2):282-9 PMID: 6321957
  2. Transformation by polyoma virus is drastically reduced by substitution of phenylalanine for tyrosine at residue 315 of middle-sized tumor antigen.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):679-83 PMID: 6322163
  3. Polyoma middle T antigen requires cooperation from another gene to express the malignant phenotype in vivo.
    Mol Cell Biol. 1984 Apr;4(4):755-60 PMID: 6325896
  4. Purification of polyoma virus.
    Virology. 1963 Feb;19:158-68 PMID: 14001211
  5. Patterns of organization of actin and myosin in normal and transformed cultured cells.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):994-8 PMID: 165499
  6. Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:637-50 PMID: 169096
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. Characterization of residual enzyme activity in fibroblasts from patients with adenosine deaminase deficiency and combined immunodeficiency: evidence for a mutant enzyme.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):213-7 PMID: 1061119
  9. Cell-to-substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique.
    J Cell Sci. 1976 Jun;21(1):129-59 PMID: 932106
  10. Correlation between tumor induction and the large external transformation sensitive protein on the cell surface.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3570-4 PMID: 1068469
  11. Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):79-83 PMID: 203944
  12. Cellular alterations dependent upon the polyoma virus Hr-t function: separation of mitogenic from transforming capacities.
    Cell. 1978 Jul;14(3):587-99 PMID: 210955
  13. Characterization of T antigens in polyoma-infected and transformed cells.
    Cell. 1978 Sep;15(1):65-77 PMID: 212199
  14. Tumor antigens induced by nontransforming mutants of polyoma virus.
    Cell. 1978 Oct;15(2):485-96 PMID: 214241
  15. Relationships between fibronectin (LETS protein) and actin.
    Cell. 1978 Nov;15(3):875-86 PMID: 365353
  16. Transport of amino acids by confluent and nonconfluent 3T3 and polyoma virus-transformed 3T3 cells growing on glass cover slips.
    J Biol Chem. 1969 May 25;244(10):2675-81 PMID: 4306034
  17. Abortive transformation by the Tsa mutant of polyoma virus.
    Nature. 1969 Jul 26;223(5204):397-8 PMID: 4309886
  18. Evidence for a functional change in the plasma membrane of murine sarcoma virus-infected mouse embryo cells. Transport and transport-associated phosphorylation of 14C-2-deoxy-D-glucose.
    J Biol Chem. 1970 Feb 25;245(4):714-7 PMID: 4313605
  19. Proteolytic enzymes initiating cell division and escape from contact inhibition of growth.
    Nature. 1970 Jul 11;227(5254):170-1 PMID: 5428405
  20. Restoration of normal growth by covering of agglutinin sites on tumour cell surface.
    Nature. 1970 Nov 7;228(5271):512-5 PMID: 5472464
  21. Behavior of tissue culture cells infected with polyoma virus.
    Proc Natl Acad Sci U S A. 1970 Nov;67(3):1214-20 PMID: 4320974
  22. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant.
    Virology. 1971 Nov;46(2):470-9 PMID: 4331732
  23. Absence of a cell membrane alteration function in non-transforming mutants of polyoma virus.
    Proc Natl Acad Sci U S A. 1970 Oct;67(2):929-34 PMID: 4332911
  24. Increased uptake of amino acids and 2-deoxy-D-glucose by virus-transformed cells in culture.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):585-9 PMID: 4335068
  25. A comparison of membrane proteins of normal and transformed cells by lactoperoxidase labeling.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):489-92 PMID: 4360946
  26. Alteration of cell-surface proteins by viral transformation and by proteolysis.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170-4 PMID: 4361679
  27. Uptake of 2-deoxyglucose by BALB-3T3 cells: changes after polyoma infection.
    Virology. 1974 Sep;61(1):223-8 PMID: 4370539
  28. Three species of polyoma virus tumor antigens share common peptides probably near the amino termini of the proteins.
    Cell. 1978 Dec;15(4):1427-37 PMID: 215327
  29. Comparisons of two early gene functions essential for transformation in polyoma virus and SV-40.
    Virology. 1979 Jul 15;96(1):205-28 PMID: 223300
  30. The roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells.
    Cell. 1979 Jul;17(3):635-43 PMID: 225037
  31. Cell-to-substratum contacts in living cells: a direct correlation between interference-reflexion and indirect-immunofluorescence microscopy using antibodies against actin and alpha-actinin.
    J Cell Sci. 1979 Jun;37:257-73 PMID: 383732
  32. Transmembrane linkage of fibronectin to intracellular actin-containing filaments in cultured human fibroblasts.
    Ann N Y Acad Sci. 1978 Jun 20;312:414-7 PMID: 291374
  33. Simian virus 40 small t antigen is not required for the maintenance of transformation but may act as a promoter (cocarcinogen) during establishment of transformation in resting rat cells.
    J Virol. 1979 Dec;32(3):979-88 PMID: 229274
  34. Modulation of the cell cycle during reversible growth inhibition of 3T3 and SV40-3T3 cells with succinylated concanavalin A.
    Exp Cell Res. 1980 Apr;126(2):311-9 PMID: 7363948
  35. Simian virus 40 small-t protein is required for loss of actin cable networks in rat cells.
    J Virol. 1980 Mar;33(3):1182-91 PMID: 6245268
  36. Viable deletion mutant in the medium and large T-antigen-coding sequences of the polyoma virus genome.
    J Virol. 1980 Mar;33(3):1215-20 PMID: 6245272
  37. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  38. Comparison of polyoma virus transcription in productively infected mouse cells and transformed rodent cell lines.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:63-75 PMID: 6253165
  39. Corrugated attachment membrane in WI-38 fibroblasts: alternating fibronectin fibers and actin-containing focal contacts.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4108-12 PMID: 6933459
  40. Alternatives for splicing: recognizing the ends of introns.
    Cell. 1980 Nov;22(2 Pt 2):324-6 PMID: 6160915
  41. Protein phosphorylated by the RSV transforming function.
    Cell. 1980 Dec;22(3):647-8 PMID: 6257393
  42. Fibronectin is not present in the focal adhesions formed between normal cultured fibroblasts and their substrata.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7318-22 PMID: 6938979
  43. Speculations on RNA splicing.
    Cell. 1981 Mar;23(3):643-6 PMID: 7226224
  44. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
    Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
  45. A transmembrane relationship between fibronectin and vinculin (130 kd protein): serum modulation in normal and transformed hamster fibroblasts.
    Cell. 1981 May;24(2):481-92 PMID: 6786758
  46. Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein.
    Nature. 1981 Aug 13;292(5824):595-600 PMID: 6265803
  47. Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site.
    Nature. 1982 Feb 4;295(5848):380-4 PMID: 7057903
  48. Association of fibronectin and vinculin with focal contacts and stress fibers in stationary hamster fibroblasts.
    J Cell Biol. 1982 Feb;92(2):398-408 PMID: 6801062
  49. Phosphorylation of vinculin by pp60src: what might it mean?
    Cell. 1982 Mar;28(3):437-8 PMID: 6280870
  50. Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):422-6 PMID: 6952193
  51. Isolation of transformation-defective host-range mutants of polyoma virus on normal mouse cells.
    Virology. 1982 Jun;119(2):310-6 PMID: 6281975
  52. Mutation causing premature termination of the polyoma virus medium T antigen blocks cell transformation.
    J Virol. 1982 Mar;41(3):1014-24 PMID: 6284958
  53. Immunofluorescent localization of the transforming protein of Rous sarcoma virus with antibodies against a synthetic src peptide.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5322-6 PMID: 6291037
  54. Role of small t antigen in the acute transforming activity of SV40.
    Cell. 1982 Sep;30(2):469-80 PMID: 6291770
  55. Fibronectins: multifunctional modular glycoproteins.
    J Cell Biol. 1982 Nov;95(2 Pt 1):369-77 PMID: 6128348
  56. Transforming genes and gene products of polyoma and SV40.
    CRC Crit Rev Biochem. 1982;13(3):215-86 PMID: 6293767
  57. Abnormal RNA splicing causes one form of alpha thalassemia.
    Cell. 1982 Jul;29(3):895-902 PMID: 7151175
  58. A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing.
    Cell. 1982 Jul;29(3):903-11 PMID: 7151176
  59. Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit beta-globin gene.
    Nature. 1983 Jan 6;301(5895):38-43 PMID: 6296682
  60. Purification of biologically active simian virus 40 small tumor antigen.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):906-10 PMID: 6302673
  61. Polyoma virus transforming protein associates with the product of the c-src cellular gene.
    Nature. 1983 Jun 2-8;303(5916):435-9 PMID: 6304524
  62. Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4 PMID: 6304690
  63. High mutation frequency in DNA transfected into mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3015-9 PMID: 6574469
  64. Host range transforming gene of polyoma virus plays a role in virus assembly.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3613-7 PMID: 6304724
  65. The hr-t gene of polyoma virus.
    Biochim Biophys Acta. 1982 Dec 21;695(2):69-95 PMID: 6305416
  66. Transforming activity of polyoma virus middle-T antigen probed by site-directed mutagenesis.
    Nature. 1983 Aug 4-10;304(5925):456-9 PMID: 6308461
  67. Role of the three polyoma virus early proteins in tumorigenesis.
    Mol Cell Biol. 1983 Aug;3(8):1451-9 PMID: 6312293
  68. T-antigen expression by polyoma mutants with modified RNA splicing.
    EMBO J. 1983;2(12):2095-101 PMID: 6321148
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.12.2774-2783.1984
Published
1984-12-00
Pages
2774-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369288
Subset
IM
Grants
NCI NIH HHS · CA 19567 · United States
NCI NIH HHS · CA 25390 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.12.2774-2783.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2