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PMID: 6096825 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Human Cu/Zn superoxide dismutase gene: molecular characterization of its two mRNA species.

Nucleic acids research ·Vol. 12 ·No. 24 ·1984-12-21 ·Pages 9349-65

Sherman L, Levanon D, Lieman-Hurwitz J, Dafni N, Groner Y

Abstract

Two cytoplasmic superoxide dismutase (SOD-1) mRNAs of about 0.7 and 0.9 kilobases (Kb.) were previously found in a variety of human cells. The two SOD-1 mRNAs are transcribed from the same gene and the major 0.7 Kb. species is approximately four times more abundant than the minor 0.9 Kb. mRNA. These two mRNAs differ in the length of their 3'-untranslated region and both have multiple 5'-ends. The longer transcript contains 222 additional nucleotides beyond the 3'-polyadenylated terminus of the short mRNA. S1 nuclease mapping and sequence analysis showed that these extra 222 nucleotides are specified by sequences contiguous to those shared by the two SOD-1 mRNAs. The 5'-termini of the two SOD-1 mRNAs were identified and mapped by both primer extension and S1 mapping. The majority of SOD-1 mRNA molecules (90-95%) have a 5'-start site located 23 base pairs (b.p.) downstream of the hexanucleotide -TATAAA-. The rest of the SOD-1 mRNA molecules have 5'-termini 30, 50 and 65 b.p. upstream from the major start region.

MeSH Terms
Base Sequence Copper Endonucleases Humans Molecular Weight Nucleic Acid Hybridization Poly A/genetics RNA, Messenger/genetics Single-Strand Specific DNA and RNA Endonucleases Superoxide Dismutase/genetics Zinc
Chemicals
RNA, Messenger Poly A Copper Superoxide Dismutase Endonucleases Single-Strand Specific DNA and RNA Endonucleases Zinc
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sherman L
Levanon D
Lieman-Hurwitz J
Dafni N
Groner Y
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.24.9349
Published
1984-12-21
Pages
9349-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320466
Subset
IM
Databases
GENBANK
X01661, X01662, X01780, X01784
External Links
PubMed source DOI: 10.1093/nar/12.24.9349 PMC Full Text View journal details
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