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PMID: 6128724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Heterogeneous initiation regions for transcription of the chicken ovomucoid gene.

Nucleic acids research ·Vol. 10 ·No. 18 ·1982-09-25 ·Pages 5553-67

Lai EC, Roop DR, Tsai MJ, Woo SL, O'Malley BW

Abstract

We have identified the 5' termini of ovomucoid RNA transcripts isolated from estrogen stimulated chick oviduct by S1-nuclease mapping. Most of the ovomucoid RNA molecules (88-89%) are initiated at a predominant region which is positioned at about 30 basepairs downstream from a "TATATAT" sequence. However, there is at least one additional initiation region which accounts for 9% of the ovomucoid RNA transcripts. It is located at approximately 80 basepairs further upstream (5') from the major initiation region. DNA sequence data reveal that an AT-rich sequence resembling the "TATA" box is also present 30 nucleotides before this minor region. Our finding provides additional insight into the molecular mechanism involved in the transcription initiation of eucaryotic genes, and indicates that in vivo synthesis of mRNA may not require an absolute site for initiation of transcription.

MeSH Terms
Animals Base Sequence Chickens Egg Proteins/genetics Estrogens/pharmacology Female Genes Nucleic Acid Hybridization Oviducts/drug effects,metabolism Ovomucin/genetics Poly A/genetics Progesterone/pharmacology RNA, Messenger/genetics Transcription, Genetic
Chemicals
Egg Proteins Estrogens RNA, Messenger Poly A Ovomucin Progesterone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lai E C
Roop D R
Tsai M J
Woo S L
O'Malley B W
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.18.5553
Published
1982-09-25
Pages
5553-67
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320906
Subset
IM
Grants
NIGMS NIH HHS · GM-29697 · United States
NICHD NIH HHS · HD-8188 · United States
Databases
GENBANK
J00894
External Links
PubMed source DOI: 10.1093/nar/10.18.5553 PMC Full Text View journal details
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