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PMID: 6095301 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a retrovirus-like repetitive element in human DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 23 ·1984-12-00 ·Pages 7510-4

Mager DL, Henthorn PS

Abstract

We describe a 5- to 6-kilobase-pair repetitive family in human DNA. One member of this family is linked to the beta-globin gene cluster and is close to the 3' breakpoints of three different naturally occurring deletions involving this gene cluster. Sequence analysis indicates that this element includes terminal direct repeats of 415 base pairs that exhibit the features of long terminal repeats (LTRs) of retroviruses. A potential histidine tRNA primer binding site occurs just 3' to the 5' direct repeat. This retrovirus-like element interrupts a member of the Kpn I family of repeated DNA and is bracketed by a 5-base-pair directly repeated sequence. When attempts are made to clone the element in bacteriophage, homologous recombination between the LTR-like sequences is very frequently observed. Copy number estimates by two methods indicate that the element is repeated 800-1000 times in the human genome. We term this Homo sapiens family of retrovirus-like elements having a histidine tRNA primer binding site the hsRTVL-H family.

MeSH Terms
Base Sequence Cells, Cultured DNA/isolation & purification DNA Restriction Enzymes DNA, Viral/isolation & purification Embryo, Mammalian Female Genes Genes, Viral Globins/genetics Humans Nucleic Acid Hybridization Pregnancy Repetitive Sequences, Nucleic Acid Retroviridae/genetics
Chemicals
DNA, Viral Globins DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mager D L
Henthorn P S
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40 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.23.7510
Published
1984-12-00
Pages
7510-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392176
Subset
IM
Grants
NIADDK NIH HHS · AM20120 · United States
NIGMS NIH HHS · GM09261 · United States
NIGMS NIH HHS · GM20069 · United States
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GENBANK
J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K01891, K01892, K02544
External Links
PubMed source DOI: 10.1073/pnas.81.23.7510 PMC Full Text View journal details
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