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PMID: 6095300 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Enhanced recombination between lambda plac5 and F42lac: identification of cis- and trans-acting factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 23 ·1984-12-00 ·Pages 7500-4

Seifert HS, Porter RD

Abstract

Enhanced transductional recombination between specialized transducing phage lambda plac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational transfer of the F sex factor of Escherichia coli. The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/genetics Genes, Viral Plasmids Protein Biosynthesis Recombination, Genetic Transduction, Genetic beta-Galactosidase/genetics
Chemicals
DNA, Recombinant DNA Restriction Enzymes beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Seifert H S
Porter R D
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.23.7500
Published
1984-12-00
Pages
7500-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392174
Subset
IM
Grants
NIGMS NIH HHS · GM-26422 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.23.7500 PMC Full Text View journal details
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