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PMID: 6219392 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Indirect stimulation of genetic recombination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 5 ·1983-03-00 ·Pages 1401-5

Golub EI, Low KB

Abstract

Recombination between lacZ alleles in crosses of lambdalacZ(1) (-) x lambdalacZ(2) (-) and F(-)lacZ(1) (-) x lambdalacZ(2) (-) in Escherichia coli (lambda) can be stimulated manyfold by UV irradiation of one of the lambdalacZ phages [Porter, R. D., McLaughlin, T. & Low, B. (1979) Cold Spring Harbor Symp. Quant. Biol. 43, 1043-1048]. Analogous stimulation has now been observed by coinfection of the cells by UV-irradiated lambda phage which carries no lac region. This indirect stimulation is not dependent on induction of the SOS system. The bacterial uvr system can effectively remove the damages on the lambda DNA which cause the indirect stimulation. Among a number of mutations tested, only ssb-1 was found to cause a drastic decrease in the indirect stimulation. Indirect stimulation was caused only by using phage that had a region of homology with the recombining phage. The homologous region can be separated from the recombining region by an extended nonhomologous region (>7.9 x 10(3) base pairs). This implies that damages to the DNA molecule, which stimulate recombination, can be located very far from the recombining region of the molecule.

MeSH Terms
Bacteriophage lambda/genetics Crosses, Genetic DNA, Viral/radiation effects Recombination, Genetic Ultraviolet Rays
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Golub E I
Low K B
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.5.1401
Published
1983-03-00
Pages
1401-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393605
Subset
IM
Grants
NIGMS NIH HHS · GM23441 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.5.1401 PMC Full Text View journal details
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