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PMID: 6095188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Identification, physical map location and sequence of the denV gene from bacteriophage T4.

Nucleic acids research ·Vol. 12 ·No. 21 ·1984-11-12 ·Pages 8085-96

Valerie K, Henderson EE, deRiel JK

Abstract

The denV gene from bacteriophage T4, which codes for endonuclease V, a small DNA repair enzyme, has been cloned and identified by an approach combining DNA sequencing and genetics, independent of the phenotypic effect of the cloned gene. Appropriate DenV+ and DenV- deletion mutants were mapped physically to define precisely a region encompassing the denV gene. This region was sequenced in order to identify a protein-coding sequence of the correct size for the denV gene (400-500 bp). Finally, identification was confirmed by sequencing the corresponding fragments cloned from four genetically and phenotypically well-characterized denV mutants. The denV gene is located at 64 kb on the T4 genome, adjacent to the ipII gene, and codes for a basic protein of 138 amino acids with a deduced molecular weight of 16,078.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes Deoxyribonuclease (Pyrimidine Dimer) Endodeoxyribonucleases/genetics Escherichia coli/enzymology,genetics,radiation effects Genes Genes, Viral Mutation T-Phages/enzymology,genetics,radiation effects Ultraviolet Rays
Chemicals
Endodeoxyribonucleases DNA Restriction Enzymes Deoxyribonuclease (Pyrimidine Dimer)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Valerie K
Henderson E E
deRiel J K
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.21.8085
Published
1984-11-12
Pages
8085-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320281
Subset
IM
Databases
GENBANK
K02201, X01124
External Links
PubMed source DOI: 10.1093/nar/12.21.8085 PMC Full Text View journal details
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