Specific action of T4 endonuclease V on damaged DNA in xeroderma pigmentosum cells in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2958-62

Tanaka K, Hayakawa H, Sekiguchi M, Okada Y

Abstract

The specific action of T4 endonuclease V on damaged DNA in xeroderma pigmentosum cells was examined using an in vivo assay system with hemagglutinating virus of Japan (Sendai virus) inactivated by UV light. A clear dose response was observed between the level of UV-induce unscheduled DNA synthesis of xeroderma pigmentosum cells and the amount of T4 endonuclease V activity added. The T4 enzyme was unstable in human cells, and its half-life was 3 hr. Fractions derived from an extract of Escherichia coli infected with T4V1, a mutant defective in the endonuclease V gene, showed no ability to restore the UV-induced unscheduled DNA synthesis of xeroderma pigmentosum cells. However, fractions derived from an extract of T4D-infected E. coli with endonuclease V activity were effective. The T4 enzyme was effective in xeroderma pigmentosum cells on DNA damaged by UV light but not in cells damaged by 4-nitroquinoline 1-oxide. The results of these experiments show that the T4 enzyme has a specific action on human cell DNA in vivo. Treatment with the T4 enzyme increased the survival of group A xeroderma pigmentosum cells after UV irradiation.

MeSH Terms

4-Nitroquinoline-1-oxide/pharmacology Cell Line Coliphages/enzymology DNA/biosynthesis,metabolism DNA Repair Endonucleases/metabolism Genes Humans Parainfluenza Virus 1, Human/radiation effects Ultraviolet Rays Xeroderma Pigmentosum/enzymology,genetics,metabolism

Chemicals

4-Nitroquinoline-1-oxide DNA Endonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tanaka K

Hayakawa H

Sekiguchi M

Okada Y

References (24)

24 references, click to expand

Xeroderma pigmentosum cells contain low levels of photoreactivating enzyme.
Proc Natl Acad Sci U S A. 1975 Jan;72(1):103-7 PMID: 1054487
Xeroderma pigmentosum cells with normal levels of excision repair have a defect in DNA synthesis after UV-irradiation.
Proc Natl Acad Sci U S A. 1975 Jan;72(1):219-23 PMID: 1054497
An altered apurinic DNA endonuclease activity in group A and group D xeroderma pigmentosum fibroblasts.
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1169-73 PMID: 1063398
Xeroderma pigmentosum: biochemical and genetic characteristics.
Annu Rev Genet. 1975;9:19-38 PMID: 1108765
Human cells repair DNA damaged by nitrous acid.
Mutat Res. 1975 Mar;27(3):407-9 PMID: 1117879
Cytotoxicity of carcinogenic aromatic amides in normal and xeroderma pigmentosum fibroblasts with different DNA repair capabilities.
J Natl Cancer Inst. 1975 Jun;54(6):1287-94 PMID: 1133846
Five complementation groups in xeroderma pigmentosum.
Mutat Res. 1975 Dec;33(2-3):327-40 PMID: 1243579
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
"Ultramicroinjection" of macromolecules or small particles into animal cells. A new technique based on virus-induced cell fusion.
J Cell Biol. 1975 Aug;66(2):292-304 PMID: 167032
Modification of cell membranes with viral envelopes during fusion of cells with HVJ (Sendai virus).
Exp Cell Res. 1975 Jul;93(2):368-78 PMID: 169133
Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus).
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4071-5 PMID: 172893
Further purification and characterization of T4 endonuclease V.
Biochim Biophys Acta. 1976 Aug 18;442(2):197-207 PMID: 182258
Repair of DNA damaged by methyl methanesulfonate in bacteriophage T4.
Biochim Biophys Acta. 1976 Aug 18;442(2):208-15 PMID: 182259
Reassembled HVJ (Sendai virus) envelopes containing non-toxic mutant proteins of diphtheria toxin show toxicity to mouse L cell.
Nature. 1977 Apr 28;266(5605):839-40 PMID: 194160
The timecourse of DNA repair replication in ultraviolet-irradiated HeLa cells.
Biochim Biophys Acta. 1973 Oct 12;324(2):206-17 PMID: 4271371
Injection of foreign substances into single cells by cell fusion.
Nature. 1974 May 31;249(456):449-50 PMID: 4365359
Cellular uptake of cyclic AMP captured within phospholipid vesicles and effect on cell-growth behaviour.
Biochim Biophys Acta. 1974 Sep 23;363(3):404-18 PMID: 4376697
Studies on the substrate specificity of the T 4 excision repair endonuclease.
Mutat Res. 1972 Jun;15(2):113-23 PMID: 4559036
Removal of bound carcinogen during DNA repair in nondividing human lymphocytes.
Cancer Res. 1972 Sep;32(9):1855-60 PMID: 4641277
Induction of unscheduled DNA synthesis by the carcinogen 7-bromomethylbenz(a)anthracene and its removal from the DNA of normal and xeroderma pigmentosum lymphocytes.
Mutat Res. 1973 Aug;19(2):231-5 PMID: 4748978
Mechanism of repair of DNA in bacteriophage. II. Inability of ultraviolet-sensitive strains of bacteriophage in inducing an enzyme activity to excise pyrimidine dimers.
J Mol Biol. 1970 Jan 28;47(2):243-55 PMID: 4907729
T4 endonuclease involved in repair of DNA.
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839-45 PMID: 5275382
Defect in DNA synthesis in skin of patients with xeroderma pigmentosum demonstrated in vivo.
Science. 1970 Jun 19;168(3938):1477-8 PMID: 5445941
DNA repair characteristics and skin cancers of xeroderma pigmentosum patients in Japan.
Cancer Res. 1977 Feb;37(2):490-5 PMID: 832273

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.7.2958

Published

1977-07-00

Pages

2958-62

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431362

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.7.2958 PMC Full Text View journal details

Analysis Services

Specific action of T4 endonuclease V on damaged DNA in xeroderma pigmentosum cells in vivo.

Contact

WeChat Official Account