"Ultramicroinjection" of macromolecules or small particles into animal cells. A new technique based on virus-induced cell fusion.

The Journal of cell biology ·Vol. 66 ·No. 2 ·1975-08-00 ·Pages 292-304

Loyter A, Zakai N, Kulka RG

Abstract

A new method is described for the introduction of macromolecules and small particles into animal cells. The first step in this procedure is the trapping of particles in ghosts of human erythrocytes. This is achieved by the gradual hemolysis of erythrocytes in the presence of the particles to be trapped. The second step is the Sendai virus-induced fusion of the ghosts containing the particles with cells. By this method, ferritin and latex spheres (diameter 0.1 mum) have been "injected" into cells.

MeSH Terms

ABO Blood-Group System Carcinoma, Hepatocellular Cell Fusion Cell Membrane/ultrastructure Coliphages Culture Techniques Cytological Techniques Cytoplasm/ultrastructure DNA Viruses Erythrocytes/physiology,ultrastructure Ferritins Fluoresceins Hemolysis Humans Latex Liver Neoplasms Microscopy, Electron Microscopy, Phase-Contrast Microspheres Parainfluenza Virus 1, Human/growth & development Serum Albumin, Bovine

Chemicals

ABO Blood-Group System Fluoresceins Latex Serum Albumin, Bovine Ferritins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Loyter A

Zakai N

Kulka R G

References (15)

15 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.66.2.292

Published

1975-08-00

Pages

292-304

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2109561

Subset

External Links

PubMed source DOI: 10.1083/jcb.66.2.292 PMC Full Text View journal details

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"Ultramicroinjection" of macromolecules or small particles into animal cells. A new technique based on virus-induced cell fusion.

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