Home LiteratureArticle Details
PMID: 6093053 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequences of the coding and flanking regions of the large ribosomal subunit RNA gene of mosquito mitochondria.

Nucleic acids research ·Vol. 12 ·No. 20 ·1984-10-25 ·Pages 7771-85

HsuChen CC, Kotin RM, Dubin DT

Abstract

We have sequenced a 1.6 kbp region of the mosquito (Aedes albopictus) mitochondrial genome containing the large ribosomal subunit ("LSU") RNA gene, and have located the ends of the gene by S1 protection analysis and by comparison with RNA sequences. The gene is preceded by a tRNAval gene and followed by genes for tRNAIeuUAG (rather than tRNAleuUAA, as in mammalian mitochondria) and an extended reading frame homologous to mammalian URF1. It is approximately 1335 residues long and is very low (17%) in G + C. The 5' half is even lower in G + C (9%), and shows little apparent homology to other LSU RNA classes. The 3' half is relatively rich (26%) in G + C and has many stretches of homology to prokaryotic and mammalian mitochondrial LSU RNA.

MeSH Terms
Aedes/genetics Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Mitochondrial/genetics Endonucleases Genes Nucleic Acid Conformation Plasmids RNA, Ribosomal/genetics Single-Strand Specific DNA and RNA Endonucleases
Chemicals
DNA, Mitochondrial RNA, Ribosomal Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
HsuChen C C
Kotin R M
Dubin D T
References (32)
32 references, click to expand
  1. Sequence and arrangement of the genes for cytochrome b, URF1, URF4L, URF4, URF5, URF6 and five tRNAs in Drosophila mitochondrial DNA.
    Nucleic Acids Res. 1984 May 11;12(9):3747-62 PMID: 6328435
  2. A cluster of four transfer RNA genes in mosquito mitochondrial DNA.
    Biochem Int. 1984 Mar;8(3):385-91 PMID: 6477610
  3. The mitochondnal genome of Aspergillus nidulans contains reading frames homologous to the human URFs 1 and 4.
    EMBO J. 1983;2(3):427-35 PMID: 11894959
  4. Methylated nucleotide content of mitochondrial ribosomal RNA from hamster cells.
    J Mol Biol. 1974 Apr 5;84(2):257-73 PMID: 4133889
  5. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  6. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  7. Fine mapping of the ribosomal RNA genes of HeLa cell mitochondrial DNA.
    J Mol Biol. 1980 Apr;138(2):411-20 PMID: 7411616
  8. Pyrimidine-specific chemical reactions useful for DNA sequencing.
    Nucleic Acids Res. 1980 Oct 24;8(20):4613-9 PMID: 7443522
  9. Methylated regions of hamster mitochondrial ribosomal RNA: structural and functional correlates.
    Nucleic Acids Res. 1981 Jan 24;9(2):323-37 PMID: 6782552
  10. The 3' terminus of the large ribosomal subunit ("17S") RNA from hamster mitochondria is ragged and oligoadenylated.
    Cell. 1981 Jan;23(1):271-8 PMID: 7214525
  11. Sequence and organization of the human mitochondrial genome.
    Nature. 1981 Apr 9;290(5806):457-65 PMID: 7219534
  12. Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes.
    Nucleic Acids Res. 1981 Jul 24;9(14):3287-306 PMID: 7024913
  13. pUR222, a vector for cloning and rapid chemical sequencing of DNA.
    Nucleic Acids Res. 1981 Aug 25;9(16):4087-98 PMID: 6272193
  14. The nucleotide sequence of the large ribosomal RNA gene and the adjacent tRNA genes from rat mitochondria.
    Nucleic Acids Res. 1981 Aug 25;9(16):4139-48 PMID: 6913863
  15. Primary and secondary structures of Escherichia coli MRE 600 23S ribosomal RNA. Comparison with models of secondary structure for maize chloroplast 23S rRNA and for large portions of mouse and human 16S mitochondrial rRNAs.
    Nucleic Acids Res. 1981 Sep 11;9(17):4303-24 PMID: 6170936
  16. Different nucleotide changes in the large rRNA gene of the mitochondrial DNA confer chloramphenicol resistance on two human cell lines.
    Nucleic Acids Res. 1981 Nov 11;9(21):5785-95 PMID: 6273808
  17. Secondary structure model for 23S ribosomal RNA.
    Nucleic Acids Res. 1981 Nov 25;9(22):6167-89 PMID: 7031608
  18. Sequence and gene organization of mouse mitochondrial DNA.
    Cell. 1981 Oct;26(2 Pt 2):167-80 PMID: 7332926
  19. The primary and secondary structure of yeast 26S rRNA.
    Nucleic Acids Res. 1981 Dec 21;9(24):6935-52 PMID: 7335496
  20. Sequence analysis and precise mapping of the 3' ends of HeLa cell mitochondrial ribosomal RNAs.
    J Mol Biol. 1982 May 5;157(1):1-19 PMID: 7108954
  21. Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome.
    J Mol Biol. 1982 Apr 25;156(4):683-717 PMID: 7120390
  22. Mitochondrial L-rRNA from Aspergillus nidulans: potential secondary structure and evolution.
    Nucleic Acids Res. 1982 Aug 11;10(15):4795-801 PMID: 6752884
  23. Identification of two erythromycin resistance mutations in the mitochondrial gene coding for the large ribosomal RNA in yeast.
    Nucleic Acids Res. 1982 Nov 11;10(21):6571-7 PMID: 6757866
  24. Drosophila mitochondrial DNA: a novel gene order.
    Nucleic Acids Res. 1982 Nov 11;10(21):6619-37 PMID: 6294611
  25. Complete DNA sequence coding for the large ribosomal RNA of yeast mitochondria.
    Nucleic Acids Res. 1983 Jan 25;11(2):339-48 PMID: 6298722
  26. Drosophila melanogaster mitochondrial DNA, a novel organization and genetic code.
    Nature. 1983 Jul 21-27;304(5923):234-41 PMID: 6408489
  27. Nucleotide sequence of a segment of Drosophila mitochondrial DNA that contains the genes for cytochrome c oxidase subunits II and III and ATPase subunit 6.
    Nucleic Acids Res. 1983 Jun 25;11(12):4211-27 PMID: 6306579
  28. The 3'-terminal region of mosquito mitochondrial small ribosomal subunit RNA: sequence and localization of methylated residues.
    Plasmid. 1983 May;9(3):307-20 PMID: 6867162
  29. Identification of the 3'-ends of the two mouse mitochondrial ribosomal RNAs. The 3'-end of 16 S ribosomal RNA contains nucleotides encoded by the gene for transfer RNALeuUUR.
    J Biol Chem. 1983 Aug 25;258(16):10104-10 PMID: 6309767
  30. Refined secondary structure models for the 16S and 23S ribosomal RNA of Escherichia coli.
    Nucleic Acids Res. 1983 Nov 11;11(21):7263-86 PMID: 6359058
  31. The "erythromycin-resistance" methylated sequence of Staphylococcus aureus ribosomal RNA.
    Plasmid. 1983 Nov;10(3):293-5 PMID: 6657779
  32. A major lysine tRNA with a CUU anticodon in insect mitochondria.
    Nucleic Acids Res. 1983 Dec 20;11(24):8659-62 PMID: 6561519
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.20.7771
Published
1984-10-25
Pages
7771-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320199
Subset
IM
Grants
NCI NIH HHS · CA-09069 · United States
NIGMS NIH HHS · GM-14957 · United States
Databases
GENBANK
X01078
External Links
PubMed source DOI: 10.1093/nar/12.20.7771 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2