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PMID: 6092846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic inactivation of topoisomerase I suppresses a defect in initiation of chromosome replication in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 195 ·No. 1-2 ·1984-00-00 ·Pages 170-4

Louarn J, Bouché JP, Patte J, Louarn JM

Abstract

A strain of Escherichia coli K12 harboring simultaneously the temperature-sensitive dnaA46 mutation and a deletion of the trp-topA-cysB region plates with the same full efficiency at 30 degrees C and 42 degrees C. We have analyzed the possible involvement of the gene coding for topoisomerase I, topA, in this suppression phenomenon. The Ts phenotype was retrieved upon introduction of a plasmid-borne DNA fragment including an active topA gene into this strain, but not upon introduction of the same fragment harboring a topA::Tn1000 insertion. Replication seems to remain DnaA-dependent in the delta (topA) strain, however, since we have been unable to introduce a dnaA::Tn10 allele. We propose either that the dnaA46 gene product is overproduced and compensates for its thermal inactivation, or that initiation at oriC demands less DnaA protein in the absence of topoisomerase I.

MeSH Terms
Alleles Base Sequence Chromosomes, Bacterial/physiology DNA Replication DNA Restriction Enzymes DNA Topoisomerases, Type I/genetics Escherichia coli/genetics Genes Genes, Bacterial Genotype Plasmids Suppression, Genetic
Chemicals
DNA Restriction Enzymes DNA Topoisomerases, Type I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Louarn J
Bouché J P
Patte J
Louarn J M
References (30)
30 references, click to expand
  1. Structure and stability of transposon 5-mediated cointegrates.
    J Mol Biol. 1982 Aug 25;159(4):557-80 PMID: 6292435
  2. Evidence for a fixed termination site of chromosome replication in Escherichia coli K12.
    J Mol Biol. 1977 Sep 25;115(3):295-314 PMID: 338909
  3. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2747-51 PMID: 6265907
  4. Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):5817-21 PMID: 6310593
  5. Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling.
    Cell. 1983 Aug;34(1):105-13 PMID: 6309403
  6. Enzymatic replication of the origin of the Escherichia coli chromosome.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12 ):7370-4 PMID: 6278471
  7. The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
    Nucleic Acids Res. 1982 Nov 25;10(22):7373-85 PMID: 6296774
  8. The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC.
    Mol Gen Genet. 1979 Jul 13;174(2):117-26 PMID: 386040
  9. Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli.
    J Bacteriol. 1977 May;130(2):577-82 PMID: 400782
  10. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  11. Origin of Escherichia coli K-12 Hfr B7.
    J Bacteriol. 1983 Feb;153(2):610-5 PMID: 6296047
  12. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  13. A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature.
    Mol Gen Genet. 1978 Jun 1;162(1):9-16 PMID: 353526
  14. The gamma delta sequence of F is an insertion sequence.
    J Mol Biol. 1978 Dec 15;126(3):347-65 PMID: 745233
  15. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.
    J Mol Biol. 1971 Feb 14;55(3):441-56 PMID: 4927942
  16. Essential role of the gyrB gene product in the transcriptional event coupled to dnaA-dependent initiation of Escherichia coli chromosome replication.
    Mol Gen Genet. 1981;183(1):134-8 PMID: 6276673
  17. Continuous synthesis of the dnaA gene product of Escherichia coli in the cell cycle.
    Mol Gen Genet. 1982;186(1):87-94 PMID: 6287172
  18. Suppression of Escherichia coli dnaA46 mutations by integration of plasmid R100.1. derivatives: constraints imposed by the replication terminus.
    J Bacteriol. 1982 Aug;151(2):657-67 PMID: 7047494
  19. Molecular cloning of the region of the terminus of Escherichia coli K-12 DNA replication.
    J Bacteriol. 1983 Feb;153(2):604-9 PMID: 6296046
  20. Identification and localization of a gene that specifies production of Escherichia coli DNA topoisomerase I.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2164-8 PMID: 6264465
  21. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  22. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  23. Identification of a sex-factor-affinity site in E. coli as gamma delta.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:135-40 PMID: 6271456
  24. Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.
    Cell. 1982 Nov;31(1):35-42 PMID: 6297751
  25. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes.
    Cell. 1982 Nov;31(1):43-51 PMID: 6297752
  26. The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.
    EMBO J. 1983;2(3):463-8 PMID: 11894964
  27. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.
    Anal Biochem. 1977 May 1;79(1-2):544-52 PMID: 68686
  28. On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation.
    J Mol Biol. 1970 Nov 14;53(3):369-87 PMID: 4924005
  29. A derivative of Tn5 with direct terminal repeats can transpose.
    J Mol Biol. 1982 Feb 25;155(2):105-20 PMID: 6281443
  30. Cloning of the gene topA encoding for DNA topoisomerase I and the physical mapping of the cysB-topA-trp region of Escherichia coli.
    Nucleic Acids Res. 1983 Mar 25;11(6):1773-90 PMID: 6300778
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332741
Published
1984-00-00
Pages
170-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332741
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