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PMID: 6300778 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of the gene topA encoding for DNA topoisomerase I and the physical mapping of the cysB-topA-trp region of Escherichia coli.

Nucleic acids research ·Vol. 11 ·No. 6 ·1983-03-25 ·Pages 1773-90

Wang JC, Becherer K

Abstract

The gene topA of Escherichia coli that encodes for DNA topoisomerase I has been cloned by a combination of genetic and radioimmunal screening. The gene has been mapped to be within a 3.4 Kb segment of the bacterial genome. The intracellular level of the enzyme in strains harboring extrachromosomal copies of topA gene increases with increasing copy number of the gene and the introduction of extrachromosomal copies of the topA gene truncated at its 3' side into a topA strain of E. coli does not significantly influence the expression of the chromosomal copy of topA. These results suggest that the expression of topA is not tightly regulated. Strains in which DNA topoisomerase I is overproduced grow significantly slower in broth and give smaller size colonies on agar plates. Physical mapping of a 20 Kb region containing cysB; topA and trp has also been carried out with a number of restriction enzymes; topA is found to be immediately adjacent to cysB and is separated from trp by a 7 Kb segment where no known gene resides.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA Topoisomerases, Type I/genetics Escherichia coli/enzymology,genetics,growth & development Genes Genes, Bacterial Radioimmunoassay
Chemicals
DNA Restriction Enzymes DNA Topoisomerases, Type I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang J C
Becherer K
References (48)
48 references, click to expand
  1. Escherichia coli K-12 mutants altered in the transport systems for oligo- and dipeptides.
    J Bacteriol. 1973 Nov;116(2):751-6 PMID: 4126826
  2. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2747-51 PMID: 6265907
  3. Physical map of a 470 x 10(3) base-pair region flanking the terminus of DNA replication in the Escherichia coli K12 genome.
    J Mol Biol. 1982 Jan 5;154(1):1-20 PMID: 6281436
  4. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  5. Suppression of promoter mutations by the pleiotropic supx mutations.
    Mol Gen Genet. 1972;117(2):91-112 PMID: 4561426
  6. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  7. Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4772-6 PMID: 337300
  8. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  9. Nucleotide sequences of the trpG regions of Escherichia coli, Shigella dysenteriae, Salmonella typhimurium and Serratia marcescens.
    J Mol Biol. 1980 Oct 5;142(4):503-17 PMID: 7007652
  10. A general method for maximizing the expression of a cloned gene.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):760-4 PMID: 370836
  11. Covalent bonds between protein and DNA. Formation of phosphotyrosine linkage between certain DNA topoisomerases and DNA.
    J Biol Chem. 1980 Jun 25;255(12):5560-5 PMID: 6155377
  12. Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4280-4 PMID: 1060106
  13. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  14. Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5244-8 PMID: 388433
  15. Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3800-4 PMID: 198805
  16. Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase.
    J Biol Chem. 1969 Jun 10;244(11):3009-18 PMID: 4306289
  17. Triornithine-resistant strains of Escherichia coli. Isolation, definition, and genetic studies.
    J Biol Chem. 1974 Jan 10;249(1):143-8 PMID: 4588690
  18. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9 PMID: 4554537
  19. Interaction between DNA and Escherichia coli protein omega. Formation of a complex between single-stranded DNA and omega protein.
    J Biol Chem. 1978 Jan 25;253(2):511-8 PMID: 338610
  20. Pilus expression in Neisseria gonorrhoeae involves chromosomal rearrangement.
    Cell. 1982 Aug;30(1):45-52 PMID: 6127165
  21. Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium.
    J Mol Biol. 1980 Oct 5;142(4):489-502 PMID: 7007651
  22. Locations of the opp and supX genes of Salmonella typhimurium and Escherichia coli.
    J Bacteriol. 1980 Aug;143(2):747-52 PMID: 7009564
  23. The use of ethidium bromide-circular DNA complexes for the fluorometric analysis of breakage and joining of DNA.
    J Mol Biol. 1971 Jan 14;55(1):75-100 PMID: 5548890
  24. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  25. Identification and localization of a gene that specifies production of Escherichia coli DNA topoisomerase I.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2164-8 PMID: 6264465
  26. ANALYSIS OF UNLINKED SUPPRESSORS OF AN O degrees MUTATION IN SALMONELLA.
    Proc Natl Acad Sci U S A. 1963 Jul;50(1):140-8 PMID: 16578535
  27. Escherichia coli DNA topoisomerase I catalyzed linking of single-stranded rings of complementary base sequences.
    Nucleic Acids Res. 1978 Oct;5(10):3811-20 PMID: 214763
  28. DNA gyrase and the supercoiling of DNA.
    Science. 1980 Feb 29;207(4434):953-60 PMID: 6243420
  29. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767-71 PMID: 200930
  30. Nucleotide sequence of Escherichia coli trpE. Anthranilate synthetase component I contains no tryptophan residues.
    J Mol Biol. 1981 Feb 15;146(1):45-54 PMID: 7021857
  31. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2.
    Methods Enzymol. 1979;68:268-80 PMID: 232215
  32. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  33. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  34. Immunological screening method to detect specific translation products.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2746-9 PMID: 96441
  35. Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.
    Cell. 1982 Nov;31(1):35-42 PMID: 6297751
  36. Interaction between DNA and an Escherichia coli protein omega.
    J Mol Biol. 1971 Feb 14;55(3):523-33 PMID: 4927945
  37. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  38. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes.
    Cell. 1982 Nov;31(1):43-51 PMID: 6297752
  39. Circular DNA forms of a bacterial sex factor.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1731-8 PMID: 5237898
  40. Nonsense mutations of the supX locus: further characterization of the supX mutant phenotype.
    Mol Gen Genet. 1973 Nov 12;126(3):191-200 PMID: 4593755
  41. Catenation and knotting of duplex DNA by type 1 topoisomerases: a mechanistic parallel with type 2 topoisomerases.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):843-7 PMID: 6262776
  42. Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities.
    Nucleic Acids Res. 1975 Sep;2(9):1459-92 PMID: 1178526
  43. Escherichia coli and Salmonella typhimurium supX genes specify deoxyribonucleic acid topoisomerase I.
    J Bacteriol. 1981 Aug;147(2):679-81 PMID: 6267017
  44. E. coli and M. luteus DNA topoisomerase I can catalyze catenation of decatenation of double-stranded DNA rings.
    Cell. 1980 Nov;22(1 Pt 1):269-76 PMID: 6253080
  45. DNA topoisomerases.
    Annu Rev Biochem. 1981;50:879-910 PMID: 6267993
  46. Physicochomecial studies on interactions between DNA and RNA polymerase. Isolation and mapping of a T7 DNA fragment containing the early promoters for Escherichia coli RNA polymerase.
    Biochemistry. 1976 Dec 28;15(26):5776-83 PMID: 795461
  47. DNA is linked to the rat liver DNA nicking-closing enzyme by a phosphodiester bond to tyrosine.
    J Biol Chem. 1981 May 25;256(10):4805-9 PMID: 6262303
  48. Gene structure in the tryptophan operon of Escherichia coli. Nucleotide sequence of trpC and the flanking intercistronic regions.
    J Mol Biol. 1980 Oct 5;142(4):519-30 PMID: 7007653
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.6.1773
Published
1983-03-25
Pages
1773-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325835
Subset
IM
Grants
NIGMS NIH HHS · GM 24544 · United States
External Links
PubMed source DOI: 10.1093/nar/11.6.1773 PMC Full Text View journal details
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