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PMID: 6090388 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Isolation and expression of the Bradyrhizobium japonicum adenylate cyclase gene (cya) in Escherichia coli.

Journal of bacteriology ·Vol. 159 ·No. 3 ·1984-09-00 ·Pages 1068-71

Guerinot ML, Chelm BK

Abstract

A 5.0-kilobase-pair HindIII fragment of Bradyrhizobium japonicum DNA containing the cya gene which encodes adenylate cyclase was isolated as an insert in pBR322, using marker rescue of the maltose-negative phenotype of an Escherichia coli cya mutant for identification. The isolated B. japonicum DNA fragment was capable of reversing the pleiotropic phenotype of cya mutations when inserted in either orientation in the HindIII site of pBR322. The complemented E. coli strains produced high levels of cyclic AMP. No sequence homology between the B. japonicum cya gene and that of E. coli was detected by hybridization analysis.

MeSH Terms
Adenylyl Cyclases/genetics Base Sequence DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Genetic Complementation Test Gram-Negative Aerobic Bacteria/enzymology,genetics Mutation Plasmids Species Specificity
Chemicals
DNA Restriction Enzymes Adenylyl Cyclases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guerinot M L
Chelm B K
References (21)
21 references, click to expand
  1. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.
    J Bacteriol. 1972 Mar;109(3):971-8 PMID: 4551758
  2. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli.
    J Gen Microbiol. 1973 Jan;74(1):77-91 PMID: 4632978
  3. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 .
    J Bacteriol. 1973 Jun;114(3):1116-24 PMID: 4576398
  4. Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase.
    J Bacteriol. 1973 Nov;116(2):582-7 PMID: 4583241
  5. Deletion of the Escherichia coli crp gene.
    J Bacteriol. 1975 Apr;122(1):338-40 PMID: 164435
  6. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  7. Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants.
    J Bacteriol. 1978 Jan;133(1):149-57 PMID: 201606
  8. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  9. The cyclic 3',5'-adenosine monophosphate receptor protein and regulation of cyclic 3',5'-adenosine monophosphate synthesis in Escherichia coli.
    Mol Gen Genet. 1978 Sep 20;165(1):47-56 PMID: 213702
  10. Regulation of hydrogen utilisation in Rhizobium japonicum by cyclic AMP.
    Biochim Biophys Acta. 1979 May 16;584(3):479-92 PMID: 222344
  11. Sequence complexities of the poly(A)-containing mRNA in uninfected soybean root and the nodule tissue developed due to the infection by Rhizobium.
    Biochim Biophys Acta. 1979 Jul 26;563(2):496-507 PMID: 572705
  12. Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments.
    Gene. 1979 Sep;7(1):79-82 PMID: 159209
  13. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  14. Molecular cloning and amplification of the adenylate cyclase gene.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4684-8 PMID: 6272270
  15. Clustering of nitrogen fixation (nif) genes in Rhizobium meliloti.
    J Bacteriol. 1982 Jan;149(1):221-8 PMID: 6274844
  16. Cyclic nucleotides in procaryotes.
    Microbiol Rev. 1981 Dec;45(4):620-42 PMID: 6276705
  17. The cya locus of Escherichia coli K12: organization and gene products.
    Mol Gen Genet. 1982;188(3):465-71 PMID: 6298576
  18. Cloning and promoter analysis of the Escherichia coli adenylate cyclase gene.
    Nucleic Acids Res. 1983 Jun 11;11(11):3451-65 PMID: 6344011
  19. Physical organization of the Bradyrhizobium japonicum nitrogenase gene region.
    J Bacteriol. 1984 Sep;159(3):857-62 PMID: 6090394
  20. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  21. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
    J Mol Biol. 1962 Jul;5:109-18 PMID: 14470099
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.3.1068-1071.1984
Published
1984-09-00
Pages
1068-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215772
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.159.3.1068-1071.1984 PMC Full Text View journal details
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