Home LiteratureArticle Details
PMID: 60740 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase.

Nucleic acids research ·Vol. 3 ·No. 6 ·1976-06-00 ·Pages 1473-86

Marcus SL, Modak MJ

Abstract

The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations were found to vary by as much as 10-fold depending upon the template-primer used to direct synthesis. Addition of KCl to reaction mixtures containing optimal divalent cation concentrations produced stimulation or inhibition of DNA synthesis which was also template-specific. DNA synthesis on the modified template poly (2'-0-methylcytidylate) was uniquely stimulated by combinations of divalent cations. With Mg++ as divalent cation, deviations from classical Michaelis-Menten kinetics of substrate saturation were observed with all template-primers tested.

MeSH Terms
Avian Leukosis Virus/enzymology Avian Myeloblastosis Virus/enzymology DNA, Viral/biosynthesis Kinetics Magnesium/pharmacology Manganese/pharmacology Polynucleotides/metabolism Potassium Chloride/pharmacology RNA-Directed DNA Polymerase/metabolism Templates, Genetic
Chemicals
DNA, Viral Polynucleotides Manganese Potassium Chloride RNA-Directed DNA Polymerase Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Marcus S L
Modak M J
References (23)
23 references, click to expand
  1. Comparative ability of RNA and DNA to prime DNA synthesis in vitro: role of sequence, sugar, and structure of template-primer.
    Biochemistry. 1975 Apr 8;14(7):1412-25 PMID: 1092334
  2. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
    J Biol Chem. 1962 Feb;237:519-25 PMID: 13861902
  3. Avian myeloblastosis virus DNA polymerase. Kinetic studies on the incorporation of noncomplementary nucleotides.
    J Biol Chem. 1975 Nov 10;250(21):8404-8 PMID: 172498
  4. Evidence for allosterism in in vitro DNA synthesis on RNA templates.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):858-62 PMID: 4132534
  5. Poly(2'-O-methylcytidylate)-oligodeoxyguanylate as a template for the ribonucleic acid directed deoxyribonucleic acid polymerase in ribonucleic acid tumor virus particles and a specific probe for the ribonucleic acid directed enzyme in transformed murine cells.
    Biochemistry. 1974 Apr 9;13(8):1632-41 PMID: 4133941
  6. RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus: a zinc metalloenzyme.
    Proc Natl Acad Sci U S A. 1974 May;71(5):2091-5 PMID: 4134617
  7. RNA-directed DNA polymerase--properties and functions in oncogenic RNA viruses and cells.
    Prog Nucleic Acid Res Mol Biol. 1974;14(0):187-334 PMID: 4135976
  8. Comparative biochemical properties of RNA-directed DNA polymerases from Rauscher murine leukemia virus and avian myeloblastosis virus.
    Cancer Res. 1974 Oct;34(10):2585-93 PMID: 4137585
  9. Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose.
    J Virol. 1974 Oct;14(4):853-9 PMID: 4138457
  10. RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1789-96 PMID: 4322157
  11. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses.
    Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507-11 PMID: 4327004
  12. Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2203-6 PMID: 4332248
  13. RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction.
    J Virol. 1972 Jan;9(1):130-42 PMID: 4333539
  14. Comparison of some reactions catalyzed by deoxyribonucleic acid polymerase from avian myeloblastosis virus, Escherichia coli, and Micrococcus luteus.
    Biochemistry. 1972 Feb 15;11(4):621-9 PMID: 4334908
  15. Purification of the DNA polymerase of avian myeloblastosis virus.
    Biochim Biophys Acta. 1971 Sep 24;246(3):365-83 PMID: 4334983
  16. Comparative studies on the template-initiator requirements of the chick-embryo DNA polymerase I and the avian-myeloblastosis-virus DNA polymerase.
    Eur J Biochem. 1974 Jan 16;41(2):253-61 PMID: 4361413
  17. Synthesis of DNA complementary to AMV RNA using E. coli polymerase I.
    Biochem Biophys Res Commun. 1974 Jan;56(1):247-55 PMID: 4362942
  18. Replication of initiated polyriboadenylic acid by mammalian low molecular weight deoxyribonucleic acid polymerase.
    J Biol Chem. 1974 Dec 10;249(23):7441-6 PMID: 4436318
  19. A new sensitive method for detecting polyadenylate in viral and other ribonucleic acids using Escherichia coli deoxyribonucleic acid polymerase I.
    J Biol Chem. 1974 Nov 25;249(22):7373-6 PMID: 4612035
  20. Full length and discrete partial reverse transcripts of globin and chorion mRNAs.
    Cell. 1975 Apr;4(4):367-78 PMID: 47272
  21. Detection of reverse transcriptase in human breast tumours with poly(Cm)-oligo(dG).
    Nature. 1975 Jul 10;256(5513):140-3 PMID: 50561
  22. Conformational aspects and reactivity of DNA. Effects of manganese and magnesium ions on interaction with DNA.
    Eur J Biochem. 1972 Sep 25;29(3):528-36 PMID: 5083508
  23. In vitro transcription of DNA from the 70S RNA of Rous sarcoma virus: identification and characterization of various size classes of DNA transcripts.
    J Virol. 1975 Nov;16(5):1220-8 PMID: 52725
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.6.1473
Published
1976-06-00
Pages
1473-86
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343006
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.6.1473 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2