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PMID: 593271 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cleavage of pm7G from mRNA 5' terminal cap structures by pyrophosphatase activity in embryonic chick lens cells.

Molecular biology reports ·Vol. 3 ·No. 6 ·1977-12-00 ·Pages 413-20

Lavers GC

Abstract

The presence of pyrophosphatase activity in embryonic lens cells which cleaves pm7G and ppGm from m7G(5')pppGm was demonstrated. It was also found that m7G(5')pppG, but not G(5')pppG, was hydrolyzed, and conversion of m7GpppG to m7G*pppG, in which the 5-membered ring of the m7G moiety is open, abolished its hydrolysis. For the caps hydrolyzed, pm7G was released only in the presence of lens cellular fraction; pm7G inhibited cap hydrolysis.

MeSH Terms
Animals Chick Embryo Lens, Crystalline/enzymology Pyrophosphatases/metabolism RNA, Messenger/metabolism Structure-Activity Relationship
Chemicals
RNA, Messenger Pyrophosphatases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lavers G C
References (20)
20 references, click to expand
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00808382
Published
1977-12-00
Pages
413-20
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00808382 View journal details
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