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PMID: 57740 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effect of bleomycin on deoxyribonucleic acid synthesis in toluene-treated Escherichia coli cells.

Antimicrobial agents and chemotherapy ·Vol. 9 ·No. 2 ·1976-02-00 ·Pages 239-46

Ross SL, Moses RE

Abstract

The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene-treated Escherichia coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin-stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I-dependent DNA synthesis increases. Bleomycin does not appear to have any effect on purified E. coli DNA polymerases I or II. Our results suggest that bleomycin causes nicking of the bacterial chromosome with subsequent DNA synthesis catalyzed by DNA polymerase I.

MeSH Terms
Bleomycin/pharmacology DNA Nucleotidyltransferases/metabolism DNA Repair/drug effects DNA, Bacterial/biosynthesis Escherichia coli/drug effects,enzymology,metabolism Time Factors Toluene/pharmacology
Chemicals
DNA, Bacterial Bleomycin Toluene DNA Nucleotidyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ross S L
Moses R E
References (31)
31 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.9.2.239
Published
1976-02-00
Pages
239-46
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC429510
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.9.2.239 PMC Full Text View journal details
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