Ultraviolet-stimulated DNA synthesis in toluenzied Escherichia coli deficient in DNA polymerase I.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 129-33

Masker WE, Hanawalt PC

Abstract

Ultraviolet (UV)-stimulated nonconservative DNA synthesis has been observed in E. coli rendered permeable to nucleoside triphosphates by exposure to toluene. This synthesis is detected in toluenized cells in which the background of UV-independent DNA synthesis is reduced by use of dnaB mutants temperature-sensitive for DNA replication and additionally deficient in DNA polymerase I. UV-stimulated nonconservative synthesis is also seen in polA dnaE mutants that are deficient in two of the three known DNA polymerases. The observed UV-stimulated repair-like synthesis requires the presence of the four deoxyribonucleoside triphosphates and ATP. This mode of synthesis appears to differ from the ATP-independent nonconservative DNA synthesis previously reported to occur in toluenized bacteria.

MeSH Terms

Adenosine Triphosphate/metabolism Bromodeoxyuridine/metabolism Carbon Isotopes Centrifugation, Density Gradient DNA Nucleotidyltransferases/metabolism DNA Repair DNA Replication DNA, Bacterial/analysis,biosynthesis Escherichia coli/drug effects,enzymology,metabolism,radiation effects Mutation Radiation Effects Temperature Thymine/metabolism Toluene/pharmacology Tritium Ultraviolet Rays Uracil Nucleotides/metabolism

Chemicals

Carbon Isotopes DNA, Bacterial Uracil Nucleotides Tritium Toluene Adenosine Triphosphate DNA Nucleotidyltransferases Bromodeoxyuridine Thymine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Masker W E

Hanawalt P C

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.1.129

Published

1973-01-00

Pages

129-33

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433199

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.1.129 PMC Full Text View journal details

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Ultraviolet-stimulated DNA synthesis in toluenzied Escherichia coli deficient in DNA polymerase I.

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