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PMID: 51933 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Biochemical properties of the bromodeoxyuridine-induced guinea pig virus.

Journal of virology ·Vol. 16 ·No. 4 ·1975-10-00 ·Pages 1039-50

Michalides R, Schlom J, Dahlberg J, Perk K

Abstract

The biophysical and biochemical properties of the virus particles released by guinea pig embryo cells treated with 5-bromo-2'-deoxyuridine (BUdR) have been compared to those of the B-type mouse mammary tumor virus (MMTV) and the C-type Rauscher murine leukemia virus. The high-molecular-weight (60 to 70S) RNA of the BUdR-induced guinea pig virus (GPV) has a molecular weight of 8 X 106 when measred by mixed agarose polyacylamide gel electrophoresis. The virus particles isolated from the tissue culture medium of BUdR-induced guniea pig cells have the following properties in common with MMTV: (i) a buoyant density of 1.18 g/ml in sucrose and 1.21 g/ml in CsCl, and (ii) a DNA polymerase that prefers Mg2+ over Mn2+ in an assay using the synthetic template poly(rC):oligo(dG). No nucleic acid sequence homology between GPV RNA and the viral RNAs of the MMTV, murine leukemia virus, hamster sarcoma virus, or Mason-Pfizer monkey virus could be observed in a competition hybridization assay using the radioactive-labeled GPV 60 to 70S RNA. By this same competition by hybridization assay the frequency of GPV proviral sequences was estimated to be at least 83 per haploid cellular genome of guniea pig cells. No nucleic acid sequences related to be GPV RNA were detected in the DNA of normal tissues of mice, rats, cats, dogs, baboons, or humans by direct RNA-DNA hybridization using radioactive GPV60 to 70S RNA.

MeSH Terms
Animals Bromodeoxyuridine/pharmacology Cells, Cultured Guinea Pigs Magnesium/pharmacology Mammary Tumor Virus, Mouse/ultrastructure Manganese/pharmacology Molecular Weight Oncogenic Viruses/enzymology,growth & development,ultrastructure RNA Viruses/growth & development,ultrastructure RNA, Viral/analysis RNA-Directed DNA Polymerase/metabolism Rauscher Virus/ultrastructure Species Specificity Virus Replication
Chemicals
RNA, Viral Manganese RNA-Directed DNA Polymerase Bromodeoxyuridine Magnesium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Michalides R
Schlom J
Dahlberg J
Perk K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.16.4.1039-1050.1975
Published
1975-10-00
Pages
1039-50
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354766
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.16.4.1039-1050.1975 PMC Full Text View journal details
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