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PMID: 4683879 Published · ppublish English Journal Article

Radioimmunoassay measurement of prostaglandins E, A, and F in human plasma.

The Journal of clinical investigation ·Vol. 52 ·No. 2 ·1973-02-00 ·Pages 398-405

Jaffe BM, Behrman HR, Parker CW

Abstract

The details of a radioimmunoassay capable of measuring as 5 pg of prostaglandin A, E, and F (PGA, PGE, and PGF) in human and rat plasma are described. Plasma samples are extracted (with 4000 cpm [(3)H] PGE(1) added for calculation of recovery) with an organic solvent system at an apparent pH of 5.8 and then chromatographed on silicic acid columns with increasing concentrations of methanol to separate PGA, PGE, and PGF. Each chromatographed sample is measured by radioimmunoassay, using the homologous antibody and tritiated marker. 40 normal individuals had mean plasma concentrations of PGA, PGE, and PGF of 1062+/-107 pg/ml, 385+/-30 pg/ml, and 141+/-15 pg/ml, respectively. Elevated PGE levels were measured in the plasma of patients with medullary carcinoma of the thyroid, carcinoid, and neuroblastoma. Treatment of rats with indomethacin decreased serum PGE levels by 67%. The radioimmunoassay appears to be of considerable experimental as well as clinical interest.

MeSH Terms
Animals Antibodies Antibody Specificity Carcinoid Tumor/blood Chromatography Humans Hydrogen-Ion Concentration Indomethacin/pharmacology Kidney/analysis Methods Neuroblastoma/blood Prostaglandins/analysis,blood Radioimmunoassay Rats Thyroid Neoplasms/blood Tritium
Chemicals
Antibodies Prostaglandins Tritium Indomethacin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jaffe B M
Behrman H R
Parker C W
References (43)
43 references, click to expand
  1. The effects of exogenous progesterone and estradiol on prostaglandin F levels in ovariectomized ewes.
    Prostaglandins. 1972 Mar;1(3):217-28 PMID: 4676669
  2. Prostaglandin F2alpha levels in peripheral sera of man.
    Prostaglandins. 1972 Jan;1(1):71-80 PMID: 4660227
  3. SUSTAINED DEPRESSOR EFFECT OF RENAL MEDULLARY EXTRACT IN THE NORMOTENSIVE RAT.
    Circ Res. 1963 Oct;13:359-66 PMID: 14072724
  4. PROSTAGLANDINS AND RELATED FACTORS. 28. METABOLISM OF PROSTAGLANDIN E1 IN GUINEA PIG LUNG: THE STRUCTURES OF TWO METABOLITES.
    J Biol Chem. 1964 Dec;239:4097-102 PMID: 14247654
  5. QUANTITATIVE DETERMINATION OF PROSTAGLANDINS IN HUMAN SEMEN.
    Clin Chim Acta. 1964 Dec;10:566-8 PMID: 14254112
  6. SYNTHESIS OF TRITIUM-LABELED PROSTAGLANDIN E-2 AND STUDIES ON ITS METABOLISM IN GUINEA PIG LUNG. 37. PROSTAGLANDINS AND RELATED FACTORS.
    J Biol Chem. 1965 May;240:1932-40 PMID: 14301783
  7. Biosynthesis of prostaglandins from arachidonic acid in guinea pig lung. Prostaglandins and related factors. 38.
    J Biol Chem. 1965 Sep;240(9):3518-21 PMID: 5837629
  8. Coated charcoal immunoassay of insulin.
    J Clin Endocrinol Metab. 1965 Oct;25(10):1375-84 PMID: 5320561
  9. The identification of prostaglandins in human umbilical cord.
    Br J Pharmacol Chemother. 1967 Feb;29(2):230-7 PMID: 6032057
  10. Prostaglandin secretion by medullary carcinoma of the thyroid. A possible cause of the associated idarrhoea.
    Lancet. 1968 Jan 6;1(7532):22-3 PMID: 4169067
  11. Pharmacologic and endocrine aspects of carcinoid syndrome.
    Adv Pharmacol. 1967;5:109-28 PMID: 4294609
  12. Prostaglandins: their disappearance from and release into the circulation.
    Nature. 1967 Dec 2;216(5118):868-73 PMID: 6074965
  13. The prostaglandins: a family of biologically active lipids.
    Pharmacol Rev. 1968 Mar;20(1):1-48 PMID: 4873508
  14. The release and fate of vaso-active hormones in the circulation.
    Br J Pharmacol. 1969 Feb;35(2):209-42 PMID: 4388134
  15. Dehydration of prostaglandins: study by spectroscopic method.
    J Lipid Res. 1969 May;10(3):320-5 PMID: 5785003
  16. Separation, identification, and estimation of prostaglandins.
    Methods Biochem Anal. 1969;17:325-71 PMID: 4894563
  17. Selective passage of prostaglandins across the lung.
    Nature. 1969 Aug 16;223(5207):742-5 PMID: 5802685
  18. Prostaglandins A1,A2 and 19-hydroxy A1; their actions on smooth muscle and their inactivation on passage through the pulmonary and hepatic portal vascular beds.
    Br J Pharmacol. 1969 Nov;37(3):705-22 PMID: 5348473
  19. Inactivation of prostaglandins by the lungs.
    Nature. 1970 Feb 14;225(5233):600-4 PMID: 4983971
  20. The separation, identification and estimation of prostaglandins in nanogram quantities by combined gas chromatography-mass spectrometry.
    Life Sci I. 1970 Sep 1;9(17):983-8 PMID: 5478404
  21. Antagonism of some smooth muscle actions of prostaglandins by polyphloretin phosphate.
    Br J Pharmacol. 1970 Jul;39(3):556-63 PMID: 4320286
  22. Antigenic activity of prostaglandins.
    Biochem Biophys Res Commun. 1970 Dec 9;41(5):1171-7 PMID: 5483622
  23. Radioimmunoassay for prostaglandins.
    Science. 1971 Feb 5;171(3970):494-6 PMID: 5539203
  24. Difference in haemodynamic response to prostaglandins A and E.
    Nature. 1971 Feb 12;229(5285):498-500 PMID: 4925213
  25. Effects of prostaglandin on ovarian steroid secretion and biosynthesis during pregnancy.
    Am J Physiol. 1971 Jul;221(1):189-93 PMID: 5555784
  26. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs.
    Nat New Biol. 1971 Jun 23;231(25):232-5 PMID: 5284360
  27. Aspirin selectively inhibits prostaglandin production in human platelets.
    Nat New Biol. 1971 Jun 23;231(25):235-7 PMID: 5284361
  28. Indomethacin and aspirin abolish prostaglandin release from the spleen.
    Nat New Biol. 1971 Jun 23;231(25):237-9 PMID: 5284362
  29. Radioimmunoassay of the F prostaglandins.
    J Clin Endocrinol Metab. 1971 Aug;33(2):171-5 PMID: 5565601
  30. Prostaglandins and glaucoma.
    Br Med J. 1971 Sep 11;3(5775):615-7 PMID: 5569982
  31. Immunochemical measurement of prostaglandin or prostaglandin-like activity from normal and neoplastic cultured tissue.
    Surg Forum. 1971;22:90-2 PMID: 5121477
  32. On the metabolism of prostaglandins E 1 and E 2 in man.
    J Biol Chem. 1971 Nov 25;246(22):6713-21 PMID: 5126221
  33. Specificities of prostaglandins B 1 , F 1 , and F 2 antigen-antibody reactions.
    J Biol Chem. 1971 Nov 25;246(22):6782-5 PMID: 5166558
  34. Serum prostaglandin A isomerase.
    Biochem Biophys Res Commun. 1971 Dec 3;45(5):1169-76 PMID: 5002702
  35. Mass spectrometric determination of picomole amounts of prostaglandins E 2 and F 2 using synthetic deuterium labeled carriers.
    Biochem Biophys Res Commun. 1971 Oct 15;45(2):519-25 PMID: 5160730
  36. Medullary thyroid carcinoma detected by serum calcitonin assay.
    Arch Surg. 1972 Apr;104(4):579-86 PMID: 5013799
  37. Thyrotropin increases prostaglandin levels in isolated thyroid cells.
    J Clin Invest. 1972 Apr;51(4):1038-42 PMID: 4622570
  38. Characterization of antibody to prostaglandin F 2a .
    Biochem Biophys Res Commun. 1972 May 26;47(4):903-9 PMID: 4112694
  39. Prostaglandins and glaucoma.
    Br Med J. 1972 Oct 28;4(5834):232 PMID: 5082560
  40. Renal concentrations of prostaglandin E in acute and chronic renal ischemia.
    Biochem Biophys Res Commun. 1972 Nov 1;49(3):799-805 PMID: 4638755
  41. Prostaglandin F2alpha levels in sera during human pregnancy.
    Prostaglandins. 1972 Apr;1(4):331-7 PMID: 4676641
  42. Ovulation blockade by aspirin or indomethacin--in vivo evidence for a role of prostaglandin in gonadotrophin secretion.
    Prostaglandins. 1972 Jan;1(1):3-20 PMID: 4660225
  43. Fatty-acid distribution in the lipid fractions of healthy persons of different age, patients with atherosclerosis and patients with idiopathic hyperlipidaemia.
    J Atheroscler Res. 1961 Jan-Feb;1:47-61 PMID: 13748547
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI107196
Published
1973-02-00
Pages
398-405
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC302269
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI107196 PMC Full Text View journal details
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