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PMID: 4894563 Published · ppublish English Comparative Study Journal Article Review

Separation, identification, and estimation of prostaglandins.

Methods of biochemical analysis ·Vol. 17 ·1969-00-00 ·Pages 325-71

Shaw JE, Ramwell PW

Abstract

暂无摘要

Keywords

Adenosine Cyclic Monophosphate Biology Endocrine System Hormones Physiology Prostaglandins--analysis Prostaglandins--pharmacodynamics

MeSH Terms

Animals Biological Assay Blood Cats Chromatography, Gas Chromatography, Ion Exchange Chromatography, Thin Layer Densitometry Hydrogen-Ion Concentration Immunochemistry Optical Rotatory Dispersion Oxidoreductases Oxygen Isotopes Prostaglandins/analysis,isolation & purification,metabolism Sheep Silver Solubility Spectrophotometry Spectrum Analysis Tritium Ultraviolet Rays

Chemicals

Oxygen Isotopes Prostaglandins Tritium Silver Oxidoreductases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Shaw J E

Ramwell P W

Other Abstracts

eng

The procedures which may be and are being used to provide a basis for the analysis of submicrogram quantitities of prostaglandins are surveyed. Discussion is focused on the following: 1) sources of standards

2) properties (effect of different pH values, effect of blood, metabolism, solubility)

3) extraction

4) detection

5) estimation (ultraviolet, optical rotatory dispersion, densitometry, radioimmunoassay, enzymatic assay, isotopic methods, bioassay)

6) separation of prostaglandins (separation of PGE, PGF, and PGA with PGB compounds, separation of PGA and PGB compounds, and separation of individual prostaglandins)

and 6) structural identification. Methods of prostaglandin analysis, with the required sensitivity for application to individual tissue and fluid specimens, are still in the developmental state. Although prostaglandins may be ubiquitous throughout the animal kingdom, no systematic study of their distribution has been made to date. Recent work has shown that PGE1 has a potent effect on the formation of 3',5' cyclic adenosine monophosphate (cyclic AMP) which is widely believed to be an intracellular intermediate in hormone action.

Article Info

Journal

Methods of biochemical analysis

Abbr.

Methods Biochem Anal

ISSN

0076-6941

DOI

10.1002/9780470110355.ch8

Published

1969-00-00

Pages

325-71

Language

English

Region

United States

NLM ID

0376644

Subset

External Links

PubMed source DOI: 10.1002/9780470110355.ch8

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