Identification of polysomal RNA in BHK cells infected by sindbis virus.

Journal of virology ·Vol. 11 ·No. 4 ·1973-04-00 ·Pages 535-43

Mowshowitz D

Abstract

Polysomes were prepared from Sindbis virus-infected BHK cells. The major species of RNA in these polysomes was identified as 26S RNA (interjacent RNA) by (i) disrupting the polysomes with EDTA; (ii) treating the infected cells with puromycin; and (iii) isolating polysomes from cells infected with a temperature-sensitive mutant that does not form nucleocapsids. Small amounts of 42S RNA and 33S RNA were also found in polysomes.

MeSH Terms

Amino Acids/metabolism Animals Carbon Isotopes Cell Line Centrifugation, Density Gradient Cricetinae Edetic Acid Electrophoresis, Polyacrylamide Gel Kidney Mutation Polyribosomes/analysis Puromycin/pharmacology RNA, Ribosomal/analysis,biosynthesis,isolation & purification RNA, Viral/analysis,biosynthesis,isolation & purification Sindbis Virus/analysis,growth & development,metabolism Temperature Tritium Uridine/metabolism

Chemicals

Amino Acids Carbon Isotopes RNA, Ribosomal RNA, Viral Tritium Puromycin Edetic Acid Uridine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Mowshowitz D

References (17)

17 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.11.4.535-543.1973

Published

1973-04-00

Pages

535-43

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355135

Subset

External Links

PubMed source DOI: 10.1128/JVI.11.4.535-543.1973 PMC Full Text View journal details

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Identification of polysomal RNA in BHK cells infected by sindbis virus.

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