Selective inhibition of the synthesis of Sindbis virion proteins by an inhibitor of chymotrypsin.

Journal of virology ·Vol. 9 ·No. 1 ·1972-01-00 ·Pages 187-8

Pfefferkorn ER, Boyle MK

Abstract

Treatment of chick embryo fibroblasts infected with Sindbis virus with TPCK, the choloromethyl ketone derivative of tosyl-phenylalanine and an inhibitor of chymotrypsin, resulted in reduced synthesis of viral structural proteins and the accumulation of a high-molecular-weight polypeptide, thought to be a precursor. The analogous inhibitor of trypsin, TLCK, the chloromethyl ketone derivative of tosyllysine, had no such effect.

MeSH Terms

Animals Arboviruses/metabolism Cells, Cultured Chick Embryo Chymotrypsin/antagonists & inhibitors Electrophoresis, Disc Fibroblasts Leucine/metabolism Molecular Weight Sindbis Virus/growth & development,metabolism Sulfones/pharmacology Toluene/pharmacology Tosyl Compounds/pharmacology Tritium Trypsin Inhibitors/pharmacology Viral Proteins/analysis,biosynthesis Virus Cultivation

Chemicals

Sulfones Tosyl Compounds Trypsin Inhibitors Viral Proteins Tritium Toluene Chymotrypsin Leucine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pfefferkorn E R

Boyle M K

References (7)

7 references, click to expand

Posttranslational cleavage of virus polypeptides in arbovirus-infected cells.
J Gen Virol. 1970 Feb;6(2):319-23 PMID: 5437471
Virus-specific proteins synthesized in cells infected with RNA+ temperature-sensitive mutants of Sindbis virus.
J Virol. 1970 Mar;5(3):329-37 PMID: 5461887
Primary gene products of an arbovirus.
Biochem Biophys Res Commun. 1969 Oct 8;37(2):369-73 PMID: 4310764
Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins.
Virology. 1969 Mar;37(3):367-76 PMID: 5777557
Direct evidence for the presence of histidine in the active center of chymotrypsin.
Biochemistry. 1963 Mar-Apr;2:252-5 PMID: 13992248
Further evidence on the formation of poliovirus proteins.
J Mol Biol. 1970 May 14;49(3):657-69 PMID: 4318205
Polypeptide cleavages in the formation of poliovirus proteins.
Proc Natl Acad Sci U S A. 1968 Sep;61(1):77-84 PMID: 4301595

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.1.187-188.1972

Published

1972-01-00

Pages

187-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356277

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.1.187-188.1972 PMC Full Text View journal details

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Selective inhibition of the synthesis of Sindbis virion proteins by an inhibitor of chymotrypsin.

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